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Proteomic technology provides physical methods with the potential to assess the composition and consistency of these complex vaccines. 2\u2010DE, combined with MS, were used to generate a proteome map of an OMV vaccine, developed to disrupt a long\u2010running outbreak of group B disease in New Zealand. Seventy four spots from the protein map were identified including the outer membrane protein (OMP) antigens: PorA, PorB, RmpM and OpcA. 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