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We have used a combined approach of cDNA cloning and 2\u2010DE with MS to identify nerve growth factor (NGF) in venoms of the Australian elapid snakes and demonstrate its neurite outgrowth activity. While a single 730 nucleotide ORF, coding for a 243 amino acid precursor protein was detected in all snakes, use of 2\u2010DE identified NGF proteins with considerable variation in molecular size within and between the different snakes. The variation in size can be explained at least in part by N\u2010linked glycosylation. It is possible that these modifications alter the stability, activity and other characteristics of the snake NGFs. Further characterisation is necessary to delineate the function of the individual NGF isoforms.<\/jats:p>","DOI":"10.1002\/pmic.200600263","type":"journal-article","created":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T07:06:36Z","timestamp":1163747196000},"page":"6554-6565","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Post\u2010translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms"],"prefix":"10.1002","volume":"6","author":[{"given":"Stephen T. 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