{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:03:11Z","timestamp":1789981391238,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2006,10,19]],"date-time":"2006-10-19T00:00:00Z","timestamp":1161216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5\u2010wk\u2010old Wistar rats and the lysates were subjected to large\u2010sized 2\u2010DE at pH 5\u20136. Among approximately 800 protein spots detected in the central and peripheral fractions, one of the unique spots in the peripheral fraction with MW of 60\u2009kDa and p<jats:italic>I<\/jats:italic> of 5.6 was identified as an isoform of collapsin response mediator protein\u20102 (CRMP\u20102) by MALDI\u2010TOF MS and Western blots with anti\u2010CRMP\u20102 antibodies that recognize 1\u201317 and 486\u2013528 residues. Since this novel spot was detected only in the peripheral fraction, but not in the central fraction, DRG, and other regions of the brain, it was named periCRMP\u20102. The C\u2010terminal fragment of CRMP\u20102 was not detected in periCRMP\u20102 by MS analyses. Expression of periCRMP\u20102 decreased following sciatic nerve injury. These results suggest that periCRMP\u20102 is a C\u2010terminal truncated isoform polarized in the peripheral side of spinal nerves and may be involved in nerve degeneration and regeneration.<\/jats:p>","DOI":"10.1002\/pmic.200600300","type":"journal-article","created":{"date-parts":[[2006,10,19]],"date-time":"2006-10-19T07:50:01Z","timestamp":1161244201000},"page":"6085-6094","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Proteomic identification of a novel isoform of collapsin response mediator protein\u20102 in spinal nerves peripheral to dorsal root 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