{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:45:02Z","timestamp":1785815102596,"version":"3.56.0"},"reference-count":42,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T00:00:00Z","timestamp":1164672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Restricting bacterial growth by iron\u2010chelating proteins that reduce iron availability in mucosal secretions and body fluids belongs to basic mechanisms of innate immunity. Most pathogens and commensals thus developed gene regulons responding to iron concentration and encoding iron acquisition systems and genes involved in host colonization and virulence. Here, we analyzed the steady\u2010state composition of the iron\u2010regulated proteome and transcriptome of an invasive serogroup\u2005C clinical isolate of <jats:italic>Neisseria meningitidis<\/jats:italic>. The proteome of meningococci grown under iron\u2010depleted and iron\u2010replete conditions was analyzed by 2\u2010DE and proteins exhibiting significantly altered expression were identified by MALDI\u2010TOF MS analysis. In parallel, total RNA was isolated from the same cultures and iron\u2010regulated genes were identified using whole\u2010genome DNA microarrays. The proteome and the transcriptome were found to overlap by only 19\u2005iron\u2010regulated genes\/proteins, with 111\u2005genes\/proteins being significantly up\u2010regulated in iron\u2010replete cultures and 130\u2005genes\/proteins being up\u2010regulated during iron starvation, respectively. Comparisons with published transcriptomic data for <jats:italic>N.\u2005meningitidis<\/jats:italic> serogroup\u2005B, moreover, indicate that expression of up to 20% of all meningococcal genes can be subject to regulation in function of iron availability.<\/jats:p>","DOI":"10.1002\/pmic.200600312","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T11:12:40Z","timestamp":1164712360000},"page":"6194-6206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The iron\u2010regulated transcriptome and proteome of <b><i>Neisseria meningitidis<\/i><\/b> serogroup\u2005C"],"prefix":"10.1002","volume":"6","author":[{"given":"Marek","family":"Basler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Linhartov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Halada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Novotn\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Bezou\u0161kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radim","family":"Osi\u010dka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslav","family":"Weiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Vohradsk\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"\u0160ebo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66956-9_1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.137"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.9.2171-2175.1987"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.35.3.915-920.1982"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.33.2.555-564.1981"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01411.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1128\/iai.18.1.94-98.1977","volume":"18","author":"Payne S. 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