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Liver cell homogenates were subfractionated by differential ultracentrifugation into cytosol and microsomes, which were subjected to 2\u2010DE to generate the proteomic maps of these fractions. 2\u2010DE yielded 1100 and 800\u2005protein spots for microsomes and cytosol, respectively. General trends of the fraction\u2010specific alterations after 3\u2010MC or PB treatment were evaluated using the Student's <jats:italic>t<\/jats:italic>\u2010test and the principal component analysis (PCA). According to the PCA\u2010derived data, the microsomal changes after 3\u2010MC and PB treatment were quite similar. However, in the case of the cytosol data, the specificities of 3\u2010MC\u2010 and PB\u2010induced responses could be clearly distinguished from each other. Protein spots, whose expression levels differed from control, were identified by MALDI\u2010TOF PMF. Proteomic studies such as those reported herein can be useful in identifying the molecular\u2010based toxicity of lead drug candidates.<\/jats:p>","DOI":"10.1002\/pmic.200600342","type":"journal-article","created":{"date-parts":[[2006,8,8]],"date-time":"2006-08-08T15:12:58Z","timestamp":1155049978000},"page":"4662-4670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Proteomic profiles of induced hepatotoxicity at the subcellular level"],"prefix":"10.1002","volume":"6","author":[{"given":"Victor","family":"Zgoda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Tikhonova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Viglinskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Serebriakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey","family":"Lisitsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Archakov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,8]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"610","volume-title":"Rat and human liver cytochromes P450: Cytochrome P450s, Structure, Mechanism, and Biochemistry","author":"Correia M. 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