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To understand the complex signaling networking associated with PP2A, we searched proteins interacting with the catalytic subunit of protein phosphatase 2A (PP2Ac) by a pull\u2010down analysis followed by 2\u2010D gel electrophoresis and proteomic analyses. The probability of identification of the proteins interacting with PP2Ac was increased by searching proteins differently interacting with PP2Ac according to stimulation of Wnt3a, which regulates both the Wnt\/\u03b2\u2010catenin and the ERK pathways. Around 100 proteins, pulled\u2010down by His\u2010tagged PP2Ac, were identified in 2\u2010D gels stained with CBB. By MALDI\u2010TOF\u2010MS analyses of 45 protein spots, we identified several proteins that were previously known to interact with PP2A, such as Axin and CaMK IV. In addition, we also identified many proteins that potentially interact with PP2Ac. The interactions of several candidate proteins, such as tuberous sclerosis complex 2, RhoB, R\u2010Ras, and Nm23H2, with PP2Ac, were confirmed by <jats:italic>in vitro<\/jats:italic> binding analyses and\/or coimmunoprecipitation experiments.<\/jats:p>","DOI":"10.1002\/pmic.200600480","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T05:34:09Z","timestamp":1165988049000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Identification of proteins interacting with the catalytic subunit of PP2A by proteomics"],"prefix":"10.1002","volume":"7","author":[{"given":"Won\u2010Jeong","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Uk","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang\u2010Yell","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1993.73.4.673"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3110017"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01375-4"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.10.110194.000415"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-005-9075-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(04)00026-1"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00535-9"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-0018-x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000639200"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003276"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5410.2089"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80140-3"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.59"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.04.007"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1101\/gad.14.8.895","volume":"14","author":"Li W.","year":"2000","journal-title":"Genes Dev."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.9.3035-3045.2002"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5383.1668"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.10.4407-4416.2004"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1743"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100443200"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203359"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.14.1768"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506986200"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411709200"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01601"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.04.125"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.01.041"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3439"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5367.1258"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404523200"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.18.7965-7975.2004"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00100-8"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3610143"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208246200"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.58.070189.002321"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00852-8"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-0364"},{"key":"e_1_2_1_39_2","first-page":"4766","volume":"58","author":"Plank T. 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