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Suppression\u2010subtractive hybridization combined with immunohistochemistry and\/or quantitative real\u2010time RT\u2010PCR were used to identify and characterize abundant stress\/inflammatory gene sequences differentially expressed in tuberculous (TB+) wild boars. Genes identified in MLN and tonsils corresponded to serum amyloid A, arginase I, osteopontin, lysozyme, annexin I, and heat shock proteins, respectively. Global protein patterns in MLN and tonsils were compared between TB+ and nontuberculous (TB\u2212) boars by 2\u2010DE and MALDI\u2010TOF MS. Five proteins, including stress\/inflammatory proteins annexin V, serum albumin, and apolipoprotein A1 were found at lower levels in MLN of TB+ boars. Manganese superoxide dismutase was found up\u2010regulated in MLN of TB+ boars. Five proteins, including creatine kinase and MHC class II antigens were found up\u2010regulated in tonsils of TB+ boars. These results demonstrated differential stress\/inflammatory responses in wild boars naturally infected with <jats:italic>M. bovis<\/jats:italic> and suggest possible markers of tuberculosis in this species that may prove useful for future studies of host\u2013pathogen interactions and for diagnostics and vaccine development.<\/jats:p>","DOI":"10.1002\/pmic.200600527","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T05:34:18Z","timestamp":1165988058000},"page":"220-231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Proteomic and transcriptomic analyses of differential stress\/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with <b><i>Mycobacterium bovis<\/i><\/b>"],"prefix":"10.1002","volume":"7","author":[{"given":"Victoria","family":"Naranjo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Villar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma Paz","family":"Mart\u00edn\u2010Hernando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolors","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"H\u00f6fle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Gortazar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine M.","family":"Kocan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.20.1.1263"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1054\/tube.2000.0263"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.431-434.2004"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1003.020819"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03548"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.152.25.779"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2004051"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.08.007"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2602-2608.2004"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005044"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2005.10.005"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2005.00703.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(03)00003-5"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.11.027"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1128\/JCM.37.9.2766-2771.1999","volume":"37","author":"Serraino A.","year":"1999","journal-title":"J. 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