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Hepatic fibrosis was induced by repetitive intraperitoneal CCl<jats:sub>4<\/jats:sub> treatment of BALB\/c mice or developed spontaneously in BALB\/c\u2010ATP\u2010binding cassette, subfamily B, member 4 (Abcb4) knock out mice. Fibrosis was verified by a morphometric score and assessment of hydroxyproline content of liver tissue, respectively. The innovative difference in\u2010gel electrophoresis (DIGE) technique was used to analyse protein expression levels of the mouse proteome. Results were confirmed by Western blotting and real\u2010time RT\u2010PCR. In CCl<jats:sub>4<\/jats:sub>\u2010induced fibrosis 20 out of 40 and in BALB\/c\u2010<jats:italic>Abcb4<\/jats:italic><jats:sup>\u2212<jats:italic>\/<\/jats:italic>\u2212<\/jats:sup> mice 8 out of 28 differentially expressed proteins were identified utilizing DIGE. Only two proteins, selenium\u2010binding protein (Sbp2) and carbonic anhydrase 3, have been unidirectionally expressed (<jats:italic>i.e.<\/jats:italic> down\u2010regulated) in both models. Relevant differences in the pathogenesis of toxically induced liver fibrosis and sclerosing cholangitis exist. The only novel protein with regard to liver fibrosis depicting a unidirectional expression pattern in both animal models was Sbp2. An explicit protein function could not be clarified yet.<\/jats:p>","DOI":"10.1002\/pmic.200600580","type":"journal-article","created":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T07:11:27Z","timestamp":1163747487000},"page":"6538-6548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Changes of the hepatic proteome in murine models for toxically induced fibrogenesis and sclerosing cholangitis"],"prefix":"10.1002","volume":"6","author":[{"given":"Corinna","family":"Henkel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Roderfeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Luise","family":"Berres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Hillebrandt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lammert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut E.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"St\u00fchler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Graf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Roeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00429-4"},{"key":"e_1_2_1_4_2","first-page":"111","volume":"74","author":"Gomez D. 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