{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:58:42Z","timestamp":1791136722681,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T00:00:00Z","timestamp":1170374400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leptomeningeal metastasis (LM) is a devastating complication occurring in 5% of breast cancer patients. However, the current \u2018gold standard\u2019 of diagnosis, namely microscopic examination of the cerebrospinal fluid (CSF), is false\u2010negative in 25% of patients at the first lumbar puncture. In a previous study, we analyzed a set of 151 CSF samples (tryptic digests) by MALDI\u2010TOF and detected peptide masses that were differentially expressed in breast cancer patients with LM. In the present study, we obtain for a limited number of samples exact masses for these peptides by MALDI\u2010FTICR MS measurements. Identification of these peptides was performed by electrospray FTICR MS after separation by nano\u2010scale LC. The database results were confirmed by targeted high mass accuracy measurements of the fragment ions in the FTICR cell. The combination of automated high\u2010throughput MALDI\u2010TOF measurements and analysis by FTICR MS leads to the identification of 17 peptides corresponding to 9 proteins. These include proteins that are operative in host\u2010disease interaction, inflammation and immune defense (serotransferrin, alpha 1\u2010antichymotrypsin, hemopexin, haptoglobin and transthyretin). Several of these proteins have been mentioned in the literature in relation to cancer. The identified proteins alpha1\u2010antichymotrypsin and apolipoprotein E have been described in relation to Alzheimer's disease and brain cancer.<\/jats:p>","DOI":"10.1002\/pmic.200600719","type":"journal-article","created":{"date-parts":[[2007,2,3]],"date-time":"2007-02-03T04:07:09Z","timestamp":1170475629000},"page":"474-481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Identification of leptomeningeal metastasis\u2010related proteins in cerebrospinal fluid of patients with breast cancer by a combination of MALDI\u2010TOF, MALDI\u2010FTICR and nanoLC\u2010FTICR 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