{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:38:13Z","timestamp":1769146693629,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,4,19]],"date-time":"2007-04-19T00:00:00Z","timestamp":1176940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The venoms of stinging insects belong to the most dangerous allergen sources and can cause fatal anaphylactic reactions. Reliable prediction of a patient's risk to anaphylactic reactions is vital, and diagnosis requires the knowledge of the relevant allergens. Recently, a new hyaluronidase \u2010like glycoprotein from\n                    <jats:italic>Vespula vulgaris<\/jats:italic>\n                    (Ves v 2b) was identified. This led us to investigate hyaluronidases and also other major allergens from\n                    <jats:italic>V. germanica<\/jats:italic>\n                    and four additional\n                    <jats:italic>Vespula<\/jats:italic>\n                    species. By MALDI\u2010Q\u2010TOF\u2010MS, the new hyaluronidase\u2010like protein was shown to be the major component of the 43\u2010kDa band in all\n                    <jats:italic>Vespula<\/jats:italic>\n                    species studied. LC\u2010ESI\u2010Q\u2010TOF\u2010MS\/MS sequencing of Ves g 2a and Ves g 2b facilitated the cloning of their cDNA. Ves v 2b and Ves g 2b turned out to be essentially identical on protein level. Whereas the less abundant \u201ca\u201d form displayed enzymatic activity, the new \u201cb\u201d homologue did not. This is probably caused by amino acid exchanges in the active site, and it raises questions about the physiological role of this protein. Sequence comparisons by MS\/MS of antigen 5 and phospholipases from\n                    <jats:italic>V. vulgaris<\/jats:italic>\n                    ,\n                    <jats:italic>germanica<\/jats:italic>\n                    ,\n                    <jats:italic>maculifrons<\/jats:italic>\n                    ,\n                    <jats:italic>pensylvanica<\/jats:italic>\n                    ,\n                    <jats:italic>flavopilosa<\/jats:italic>\n                    and\n                    <jats:italic>squamosa<\/jats:italic>\n                    revealed the latter as a taxonomic outlier and led to the discovery of several not previously reported amino acid differences.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200600800","type":"journal-article","created":{"date-parts":[[2007,4,19]],"date-time":"2007-04-19T06:27:36Z","timestamp":1176964056000},"page":"1615-1623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A proteomic study of the major allergens from yellow jacket venoms"],"prefix":"10.1002","volume":"7","author":[{"given":"Daniel","family":"Kolarich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Loos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renaud","family":"L\u00e9onard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Mach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gorji","family":"Marzban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Hemmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Altmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1984.tb00876.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:30:2:109"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.01897.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.120013"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04841.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906010687"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(84)90094-0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(93)90014-7"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.4049\/jimmunol.150.7.2823","volume":"150","author":"Lu G.","year":"1993","journal-title":"J. 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