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Previously we have shown that the protein profiles and lipooligosaccharide (LOS) structures from various strains of <jats:italic>H. ducreyi<\/jats:italic> are generally well conserved. Previous studies have demonstrated that at least one strain, 33921, has a variant protein profile and LOS structure. In this study, both the whole cell lysate and the membrane proteins from strain 33921 were further examined and compared to the prototypical strain 35000HP by 2\u2010DE and by the 16\u2010BAC (benzyldimethyl\u2010<jats:italic>n<\/jats:italic>\u2010hexadecylammonium chloride)\/SDS\u2010PAGE two\u2010detergent system, respectively. These comparisons demonstrated that a number of the proteins that could be identified from both strains had altered positions on the gels, both in their apparent molecular weight and p<jats:italic>I<\/jats:italic> values. Strain 33921 has been suggested to be a member of a second class of strains, termed class II strains. In this study, the proteomic profiles and the LOS structures from the five potential class II strains were examined and found to be similar to strain 33921.<\/jats:p>","DOI":"10.1002\/pmic.200600830","type":"journal-article","created":{"date-parts":[[2007,8,6]],"date-time":"2007-08-06T06:16:25Z","timestamp":1186380985000},"page":"3131-3142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Proposed second class of <b><i>Haemophilus ducreyi<\/i><\/b> strains show altered protein and lipooligosaccharide profiles"],"prefix":"10.1002","volume":"7","author":[{"given":"Deborah M. 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