{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:53:13Z","timestamp":1791312793026,"version":"4.3.1"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,4,19]],"date-time":"2007-04-19T00:00:00Z","timestamp":1176940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The eradication of smallpox by vaccination with vaccinia virus was probably one of the greatest achievements of vaccinology. However, the immunological basis of this protection is not fully understood. To this end, we have used protein microarrays of the vaccinia (Western Reserve, WR) proteome to profile antibody reactivities after primary infection or boosting with the licensed smallpox vaccine, Dryvax\u00ae, and with archival convalescent smallpox sera. Some 25 antigens were consistently recognized by Dryvax\u00ae sera, of which half were envelope proteins (notably, H3, A13, B5, and D8). The remainder consisted mainly of core proteins (\n                    <jats:italic>e.g<\/jats:italic>\n                    . A10, L4, and I1), proteins involved in intracellular morphogenesis (A11, D13), and the A\u2010type inclusion protein, WR148. Convalescent smallpox sera also detected vaccinia antigens on the array, consistent with the notion that there is serological cross\u2010reactivity between these two orthopox species that underlies protection. Moreover, the profiles of immunodominant antigens recognized by variola\u2010infected individuals and Dryvax\u00ae vaccinees were indistinguishable. This is the first description of antibody\u2010specificity profiles induced after smallpox infection. The array data indicate that a significant component of the antibody response is not involved in virus neutralization, although these antigens should be considered alongside the envelope proteins as potential candidates for diagnostic and vaccine applications.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200600926","type":"journal-article","created":{"date-parts":[[2007,4,19]],"date-time":"2007-04-19T06:32:05Z","timestamp":1176964325000},"page":"1678-1686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":145,"title":["Proteome\u2010wide analysis of the serological response to vaccinia and smallpox"],"prefix":"10.1002","volume":"7","author":[{"given":"D. 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