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Thus, the analysis of alternative protein phosphorylation states which occur during oxidative stress\u2010induced cell death could provide valuable information regarding cell death. In this study, a reference phosphoproteome map of the mouse hippocampal cell line HT22 was constructed based on 125 spots that were identified by MALDI\u2010TOF or LC\u2010ESI\u2010Q\u2010TOF\u2010MS analysis. In addition, proteins of HT22 cells at various stages of oxidative stress\u2010induced cell death were separated by 2\u2010DE and alterations in phosphoproteins were detected by Pro\u2010Q Diamond staining. A total of 17 spots showing significant quantitative changes and seven newly appearing spots were identified after glutamate treatment. Splicing factor 2, peroxiredoxin 2, S100 calcium binding protein A11, and purine nucleoside phosphorylase were identified as up\u2010 or down\u2010regulated proteins. CDC25A, caspase\u20108, and cyp51 protein appeared during oxidative stress\u2010induced cell death. The data in this study from phosphoproteomic analysis provide a valuable resource for the understanding of HT22 cell death mechanisms mediated by oxidative stress.<\/jats:p>","DOI":"10.1002\/pmic.200601028","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T10:48:22Z","timestamp":1183459702000},"page":"2624-2635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Phosphoproteomic analysis of neuronal cell death by glutamate\u2010induced oxidative stress"],"prefix":"10.1002","volume":"7","author":[{"given":"Tae Hyuk","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang\u2010Hee","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min\u2010jung","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won\u2010Kon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyang\u2010Ran","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeyun","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phil Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunghyun","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae\u2010Sung","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Goo","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Eon","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Chul","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81004-1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.36.040196.000503"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410380304"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOBB.0000041755.22613.8d"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(02)00037-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00302-6"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-20-06394.1996"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568026013394741"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.71010095.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600644"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000071748.48338.25"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.02255.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10540-005-2846-0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90405-0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401289"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(02)00009-1"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M400054-MCP200"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4911fje"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300434"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0302-301"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1005"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T300006-MCP200"},{"key":"e_1_2_1_24_2","first-page":"249","volume":"15","author":"Chen Z.","year":"2004","journal-title":"J. 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