{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:29:20Z","timestamp":1777379360600,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2007,10,25]],"date-time":"2007-10-25T00:00:00Z","timestamp":1193270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parasitic organisms are incapable of <jats:italic>de novo<\/jats:italic> fatty acid synthesis due to a down\u2010regulated expression of enzymes involved in the oxygen\u2010dependent pathway. We investigated the uptake of host lipids by a 150\u2010kDa hydrophobic ligand\u2010binding protein (HLBP) of <jats:italic>Taenia solium<\/jats:italic> metacestode, an agent causative of neurocysticercosis. The protein was found to be a hetero\u2010oligomeric complex consisting of multiple subunits (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> 7, 10, and 15\u2005kDa within pH\u20058.0\u20139.7), which may originate from four unique genes of 7\u2010 and 10\u2010kDa gene families with 2\u20133 polymorphic alleles\/paralogs. The 15\u2010kDa protein represented glycosylated forms of the 10\u2010kDa. With high binding affinity to lipid analogs, these subunits evidenced high\u2010level sequence identity with other cestode HLBPs and form a novel clade associated with excretory\u2013secretory type HLBP. <jats:italic>In vitro<\/jats:italic> experiments with viable worms suggested that the excreted 150\u2010kDa protein might bind to lipids, and participate in the translocation of host lipids across the syncytial membrane. This process was substantially inhibited by the specific anti\u2010150\u2005kDa antibodies. The protein was localized in the parasite syncytium and in the lipid droplets within host granuloma wall, where significant lipase activity was expressed. HLBP\u2010mediated uptake of the host lipid may be critical for the parasite survival and thus could be targeted by chemotherapeutics and\/or vaccine.<\/jats:p>","DOI":"10.1002\/pmic.200700332","type":"journal-article","created":{"date-parts":[[2007,10,25]],"date-time":"2007-10-25T09:48:30Z","timestamp":1193305710000},"page":"4016-4030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["A hydrophobic ligand\u2010binding protein of the <b><i>Taenia solium<\/i><\/b> metacestode mediates uptake of the host lipid: Implication for the maintenance of parasitic cellular homeostasis"],"prefix":"10.1002","volume":"7","author":[{"given":"Eung\u2010Goo","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon\u2010Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010An","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon\u2010Yong","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myungkoo","family":"Suh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byoung\u2010Kuk","family":"Na","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong\u2010Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Insug","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,25]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Neurocysticercosis: A Clinical Handbook","author":"Del Brutto O. 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