{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T01:02:33Z","timestamp":1768352553209,"version":"3.49.0"},"reference-count":59,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2007,9,19]],"date-time":"2007-09-19T00:00:00Z","timestamp":1190160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bacillus anthracis<\/jats:italic> is a gram\u2010positive bacterial organism responsible for anthrax. This organism has two pathogenic plasmids: pX01 and pX02. The genetic function of pX01, which comprises about 198\u2005kb, is not known, except for a region called the pathogenic island, which contains three genes\u2014<jats:italic>pag<\/jats:italic>, <jats:italic>lef<\/jats:italic>, and <jats:italic>cya\u2014<\/jats:italic>that code for three toxic proteins. A 2\u2010D difference gel electrophoresis (2\u2010D DIGE) system was used to verify the existence of proteins controlled by the pX01 plasmid, and protein regulation data were obtained using DeCyder software. A total of 1728 proteins were identified in the wild\u2010type strain of this organism and 1684 in the pX01 plasmid. Twenty\u2010seven of these proteins disappeared and eight appeared when the pX01 plasmid was removed. An additional 52 proteins were downregulated and 15 were upregulated when this plasmid was removed. A total of 102 proteins have been identified using the MALDI\u2010TOF method of analysis, including 49 whose functions are unknown. Among these, 31 participate in metabolic processes, two in cellular processes, 15 in the processing of genetic information, and five in the processing of extracellular information. Another seven proteins participate in bacterial virulence and pathogenesis. We investigated the functions of these proteins in other bacteria, particularly the <jats:italic>B. anthracis<\/jats:italic> derivative H9041. Bacterial growth differed between pX01+\/pX02+ <jats:italic>B. anthracis<\/jats:italic> and its pX01\u2013\/pX02+ derivative as did the cytotoxicity of macrophages infected by pX01+\/pX02+ <jats:italic>B. anthracis<\/jats:italic> and the pX01\u2013pX02+ derivative. We also found that S100B protein levels increased in the host infected with pX01+\/pX02+ <jats:italic>B. anthracis<\/jats:italic> or its pX01\u2013\/pX02+ derivative. These data suggest that the pX01 plasmid plays a key role in the regulation of protein functions in <jats:italic>B. anthracis.<\/jats:italic><\/jats:p>","DOI":"10.1002\/pmic.200700338","type":"journal-article","created":{"date-parts":[[2007,9,19]],"date-time":"2007-09-19T14:47:05Z","timestamp":1190213225000},"page":"3743-3758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Differential analysis of <b><i>Bacillus anthracis<\/i><\/b> after pX01 plasmid curing and comprehensive data on <b><i>Bacillus anthracis<\/i><\/b> infection in macrophages and glial cells"],"prefix":"10.1002","volume":"7","author":[{"given":"Sung\u2010Ha","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee\u2010Bok","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won\u2010Keun","family":"Seong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan\u2010Wha","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u2010Yun","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheon\u2010Kwon","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01485.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90439-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.39.1.371-376.1983"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90335-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.49.2.291-297.1985"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01710.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.2.722-730.1989"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.17.5329-5338.1993"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02340.x"},{"key":"e_1_2_1_11_2","first-page":"277","volume-title":"The anthrax toxin complex. In: Sourcebook of Bacterial Protein Toxins","author":"Leppla S. H.","year":"1991"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1128\/iai.65.8.3091-3099.1997","article-title":"The anthrax toxin activator gene atxA is associated with CO2\u2010enhanced nontoxin gene expression in Bacillus anthracis","volume":"65","author":"Hoffmaster A. R.","year":"1997","journal-title":"Infect. Immun."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.3412\/jsb.51.833"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(91)90053-D"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.1.164-178.2004"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00883.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.01.007"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401218"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3646-3658.2005"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.162.2.543-550.1985"},{"key":"e_1_2_1_21_2","first-page":"349","volume-title":"Bacillus. Manual of Clinical Microbiology","author":"Turnbull P. C.","year":"1995"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3041667.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0944-5013(11)80054-6"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200103)1:3<377::AID-PROT377>3.0.CO;2-6"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300439"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200390006"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500508102"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.2.370-380.2002"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.37.100183.001523"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114533"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4895-4902.1998"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.23.6448-6456.2002"},{"key":"e_1_2_1_35_2","first-page":"418","volume-title":"Complement Binding to Bacillus anthracis. Mutants Lacking Surface Structures","author":"Ray K. C., M. S.","year":"1998"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.18.4988-5000.2002"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(93)90198-B"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)91458-9"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(83)90040-3"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.18.10412"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03800.x"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi027385d"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1830-1834.2001"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200312166"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505332200"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.5.719-724.2002"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04178.x"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzi011"},{"key":"e_1_2_1_49_2","doi-asserted-by":"crossref","first-page":"2932","DOI":"10.1128\/aem.62.8.2932-2939.1996","article-title":"The Bacillus thuringiensis insecticidal toxin binds biotin\u2010containing proteins","volume":"62","author":"Du C.","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2004.03.001"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.06.038"},{"key":"e_1_2_1_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313948200"},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-1953-y"},{"key":"e_1_2_1_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.17.4704-4710.2000"},{"key":"e_1_2_1_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20584"},{"key":"e_1_2_1_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.20.7136"},{"key":"e_1_2_1_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)91681-7"},{"key":"e_1_2_1_58_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.6.3063"},{"key":"e_1_2_1_59_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.5.2543"},{"key":"e_1_2_1_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006993200"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200700338","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200700338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:51:02Z","timestamp":1761054662000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200700338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":59,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/pmic.200700338"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200700338","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]},"assertion":[{"value":"2007-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}