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We used a sensitive 2\u2010D DIGE saturation labeling approach including an internal pooled standard for quantitative proteome profiling of immature <jats:italic>versus in vitro<\/jats:italic> matured bovine oocytes in six independent samples. The study comprised 48 2D gel images representing 24 DIGE experiments. From 250\u2005ng sample analyzed <jats:italic>per<\/jats:italic> gel, quantitative analysis revealed an average of 2244 spots in pH\u20054\u20137 images and 1291 spots in pH\u20056\u20139 images. Thirty\u2010eight spots with different intensities were detected in total. Spots of a preparative gel from 2200 oocytes were identified by nano\u2010LC\u2010MS\/MS analysis. The ten spots which could be unambiguously identified include the Ca<jats:sup>2+<\/jats:sup>\u2010binding protein translationally controlled tumor protein, enzymes of the Krebs and pentose phosphate cycles, clusterin, 14\u20103\u20103 \u03f5, elongation factor\u20101 gamma, and redox enzymes such as polymorphic forms of GST Mu 5 and peroxiredoxin\u20103. The cellular distribution of two proteins was determined by confocal laser scanning microscopy. The interesting protein candidates identified by this study may help to improve the <jats:italic>in vitro<\/jats:italic> maturation process in order to increase the rate of successful <jats:italic>in vitro<\/jats:italic> fertilization and other ART in cattle and other mammals.<\/jats:p>","DOI":"10.1002\/pmic.200700417","type":"journal-article","created":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T07:01:19Z","timestamp":1231484479000},"page":"550-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Highly sensitive saturation labeling reveals changes in abundance of cell cycle\u2010associated proteins and redox enzyme variants during oocyte maturation <b><i>in vitro<\/i><\/b>"],"prefix":"10.1002","volume":"9","author":[{"given":"Frank J.","family":"Berendt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Fr\u00f6hlich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Bolbrinker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Boelhauve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuna","family":"G\u00fcng\u00f6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix A.","family":"Habermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg J.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2004.09.010"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308487101"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.028472"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199708)47:4<456::AID-MRD13>3.0.CO;2-U"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00336.x"},{"key":"e_1_2_1_7_2","first-page":"453","article-title":"Gene expression during oogenesis and oocyte development in mammals","volume":"1","author":"Bachvarova R.","year":"1985","journal-title":"Dev. 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