{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:38:28Z","timestamp":1791038308693,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2007,11,19]],"date-time":"2007-11-19T00:00:00Z","timestamp":1195430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The genome of the obligate intracellular bacteria <jats:italic>Chlamydia pneumoniae<\/jats:italic> contains 21 genes encoding polymorphic membrane proteins (Pmp). While no function has yet been attributed to the Pmps, they may be involved in an antigenic variation of the <jats:italic>Chlamydia<\/jats:italic> surface. It has previously been demonstrated that Pmp10 is differentially expressed in the <jats:italic>C. pneumoniae<\/jats:italic> CWL029 isolate. To evaluate whether the absence of Pmp10 in the outer membrane causes further changes to the <jats:italic>C. pneumoniae<\/jats:italic> protein profile, we subcloned the CWL029 isolate and selected a clone with minimal Pmp10 expression. Subsequently, we compared the proteome of the CWL029 isolate with the proteome of the subcloned strain and identified a specific cleavage of the C\u2010terminal part of the major outer membrane protein (MOMP), which occurred only in the absence of Pmp10. In contrast, when Pmp10 was expressed we predominantly observed full\u2010length MOMP. No other proteins appeared to be regulated according to the presence or absence of Pmp10. These results suggest a close association between MOMP and Pmp10, where Pmp10 may protect the C\u2010terminal part of MOMP from proteolytic cleavage.<\/jats:p>","DOI":"10.1002\/pmic.200700447","type":"journal-article","created":{"date-parts":[[2007,11,19]],"date-time":"2007-11-19T10:46:57Z","timestamp":1195469217000},"page":"4477-4487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proteolytic cleavage of the <b><i>Chlamydia pneumoniae<\/i><\/b> major outer membrane protein in the absence of Pmp10"],"prefix":"10.1002","volume":"7","author":[{"given":"Nicolai","family":"Juul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evy","family":"Timmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Gevaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunna","family":"Christiansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svend","family":"Birkelund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,12,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.1.143-190.1991"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.2741\/hahn"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.31.3.1161-1176.1981"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/iai.44.2.306-314.1984","article-title":"Monoclonal antibody against a genus\u2010specific antigen of Chlamydia species: Location of the epitope on chlamydial lipopolysaccharide","volume":"44","author":"Caldwell H. 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