{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:05:01Z","timestamp":1791158701676,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,12,17]],"date-time":"2007-12-17T00:00:00Z","timestamp":1197849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this work, we present, to our knowledge, the first demonstration of the utility of iron oxide magnetic microspheres coated with gallium oxide for the highly selective enrichment of phosphopeptide prior to mass spectrometric analysis. These microspheres that we prepared not only have a shell of gallium oxide, giving them a high\u2010trapping capacity for the phosphopeptides, but also their magnetic property enables easy isolation by positioning an external magnetic field. Tryptic digest products of phosphoproteins including \u03b2\u2010casein, ovalbumin, casein, as well as five protein mixtures were used as the samples to exemplify the feasibility of this approach. In very short time (only 0.5\u2005min), phosphopeptides sufficient for characterization by MALDI\u2010TOF\u2010MS were selectively enriched by the Ga<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010coated Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microspheres. The performance of the Ga<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010coated Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microspheres were further compared with Fe<jats:sup>3+<\/jats:sup>\u2010immobilized magnetic silica microspheres, commercial Fe<jats:sup>3+<\/jats:sup>\u2010IMAC resin, and TiO<jats:sub>2<\/jats:sub> beads for enrichment of peptides originating from tryptic digestion of \u03b2\u2010casein and BSA with a molar ratio of 1:50, and the results proved a stronger selective ability of Ga<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010coated Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microspheres over the other materials. Finally, the Ga<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010coated Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microspheres were successfully utilized for enrichment of phosphopeptides from digestion products of rat liver extract. All results show that Ga<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010coated Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microsphere is an effective material for selective isolation and concentration of phosphopeptides.<\/jats:p>","DOI":"10.1002\/pmic.200700454","type":"journal-article","created":{"date-parts":[[2007,12,17]],"date-time":"2007-12-17T05:16:20Z","timestamp":1197868580000},"page":"238-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Highly selective and rapid enrichment of phosphorylated peptides using gallium oxide\u2010coated magnetic microspheres for MALDI\u2010TOF\u2010MS and nano\u2010LC\u2010ESI\u2010MS\/MS\/MS analysis"],"prefix":"10.1002","volume":"8","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaqing","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(98)00056-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(01)02247-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(02)02179-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81688-8"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(00)00250-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(02)01944-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar020143l"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)83005-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr990076h"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"7304","DOI":"10.1016\/S0021-9258(17)37284-8","article-title":"Expression, purification, crystallization, and preliminary\u2010X\u2010Ray analysis of casein kinase\u20101 from schizosaccharomyces \u2010pome","volume":"269","author":"Carmel G.","year":"1994","journal-title":"J. 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