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Anoxia was obtained by depleting the medium of O<jats:sub>2<\/jats:sub> by flushing with N<jats:sub>2<\/jats:sub>, and protein changes were studied by 2\u2010DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up\u2010regulated compared to the control situation and 11 were down\u2010regulated. Using MS\/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up\u2010regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up\u2010regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8\u2005h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from rainbow trout.<\/jats:p>","DOI":"10.1002\/pmic.200700460","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T10:16:39Z","timestamp":1201774599000},"page":"1009-1018","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Long term anoxia in rainbow trout investigated by 2\u2010DE and MS\/MS"],"prefix":"10.1002","volume":"8","author":[{"given":"Tune","family":"Wulff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flemming","family":"Jessen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Roepstorff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Else K.","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,3,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200524408"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63747-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(02)00132-0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-326X(02)00061-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000130856.31468.47"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1600832"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2004.0378.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0340097"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0528759"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00818.2003"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401002"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2005.01.005"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00025.2006"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"R1755","DOI":"10.1152\/ajpregu.2001.281.6.R1755","article-title":"Recovery of trout myocardial function following anoxia: Preconditioning in a non\u2010mammalian model","volume":"281","author":"Gamperl A. 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