{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T18:35:58Z","timestamp":1778956558585,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,6,12]],"date-time":"2009-06-12T00:00:00Z","timestamp":1244764800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["48004847-300"],"award-info":[{"award-number":["48004847-300"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Clonorchis sinensis<\/jats:italic>, the Chinese liver fluke, is the causative agent of clonorchiasis as well as liver and biliary diseases. The excretory\u2010secretory products (ESPs) of the parasites play important roles in host\u2013parasite interactions. In this study, we have investigated the proteome of ESPs obtained from <jats:italic>C. sinensis<\/jats:italic> adult worms. Although the full genome database of <jats:italic>C. sinensis<\/jats:italic> is not yet available, we have successfully identified 62 protein spots using 2\u2010DE\u2010based mass analysis and EST database of <jats:italic>C. sinensis<\/jats:italic>. The proteins identified include detoxification enzymes, such as glutathione <jats:italic>S<\/jats:italic>\u2010transferase and thioredoxin peroxidase, myoglobin and a number of cysteine proteases that are expressed abundantly. In order to identify potential targets for the diagnosis and therapy of clonorchiasis, we conducted immunoblot analysis of the ESPs proteome using the sera obtained from clonorchiasis patients and identified legumains and cysteine proteases as antigens present in the ESPs. Although the cysteine proteases were previously reported to elicit antigenicity, the legumains are found herein for the first time as a serological antigen of <jats:italic>C. sinensis<\/jats:italic>. To confirm these findings, we expressed recombinant legumain in <jats:italic>Escherichia coli<\/jats:italic> and verified that recombinant legumain also functions as a potent antigen against the sera of clonorchiasis patients. Our results illustrate the validity of immuno\u2010proteomic approaches in the identification of serodiagnostic antigens in the parasites.<\/jats:p>","DOI":"10.1002\/pmic.200700613","type":"journal-article","created":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T14:10:10Z","timestamp":1245075010000},"page":"3066-3078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory\u2010secretory products of <i>Clonorchis sinensis<\/i> adult worms"],"prefix":"10.1002","volume":"9","author":[{"given":"Jung\u2010Won","family":"Ju","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun\u2010Na","family":"Joo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myoung\u2010Ro","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Hyeong","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeng\u2010Il","family":"Cheun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Yeon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Hee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang\u2010Jun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woon\u2010Mok","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Min","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il\u2010Chul","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byoung Chul","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong\u2010Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,6,12]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"The prevalence of intestinal parasitic infection in Korea Korean Centers for Disease Control and Prevention and Korea Association of Health Promotion2004."},{"key":"e_1_2_6_3_2","first-page":"1114","article-title":"Investigation on the epidemiological factors of Clonorchis sinensis infection in an area of south China","volume":"36","author":"Lin R.","year":"2005","journal-title":"Southeast Asian J. 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