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To determine the effects of HBV replication on host cell\u2010protein expression, we utilized 2\u2010DE and MS\/MS analysis to compare and identify differentially expressed proteins between an HBV\u2010producing cell line HepG2.2.15 and its parental cell line HepG2. Of the 66 spots identified as differentially expressed (\u00b1\u2005over twofold, <jats:italic>p<\/jats:italic> &lt;0.05) between the two cell lines, 62 spots (corresponding to 61 unique proteins) were positively identified by MS\/MS analysis. These proteins could be clearly divided into three major groups by cluster and metabolic\/signaling pathway analysis: proteins involved in retinol metabolism pathway, calcium ion\u2010binding proteins, and proteins associated with protein degradation pathways. Other proteins identified include those that function in diverse biological processes such as signal transduction, immune regulation, molecular chaperone, electron transport\/redox regulation, cell proliferation\/differentiation, and mRNA splicing. In summary, we profiled proteome alterations between HepG2.2.15 and HepG2 cells. The proteins identified in this study would be useful in revealing the mechanisms underlying HBV\u2010host cell interactions and the development of HCC. This study can also provide some useful clues for antiviral research.<\/jats:p>","DOI":"10.1002\/pmic.200700849","type":"journal-article","created":{"date-parts":[[2008,5,19]],"date-time":"2008-05-19T11:17:24Z","timestamp":1211195844000},"page":"2012-2023","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Proteomic analysis of cellular protein alterations using a hepatitis B virus\u2010producing cellular model"],"prefix":"10.1002","volume":"8","author":[{"given":"Aiping","family":"Tong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingsong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quek Choon","family":"Lau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Canhua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010quan","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra031087"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.1.51-68.2000"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(92)90138-F"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020681"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510380633"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1054\/drup.2001.0190"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/095632020101200101"},{"key":"e_1_2_1_9_2","volume-title":"The Molecular Biology of Hepatitis B Viruses","author":"Ganem D.","year":"2000"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M600449-MCP200","article-title":"Proteome analysis of hepatocellular carcinoma by two\u2010dimensional difference gel electrophoresis","author":"Sun W.","year":"2007","journal-title":"Mol. 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