{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T08:09:44Z","timestamp":1778054984235,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T00:00:00Z","timestamp":1235692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The proteome of <jats:italic>Tropheryma whipplei<\/jats:italic>, the intracellular bacterium responsible for Whipple's disease (WD), was analyzed using two complementary approaches: 2\u2010DE coupled with MALDI\u2010TOF and SDS\u2010PAGE with nanoLC\u2010MS\/MS. This strategy led to the identification of 206 proteins of 808 predicted ORFs, resolving some questions raised by the genomic sequence of this bacterium. We successfully identified antibiotic targets and proteins with predicted N\u2010terminal signal sequences. Additionally, we identified a family of surface proteins (known as <jats:italic>T. whipplei<\/jats:italic> surface proteins (WiSPs)), which are encoded by a unique group of species\u2010specific genes and serve as both coding regions and DNA repeats that promote genomic recombination. Comparison of the protein expression profiles of the intracellular facultative host\u2010associated WD bacterium with other host\u2010associated, intracellular obligate, and environmental bacteria revealed that <jats:italic>T. whipplei<\/jats:italic> shares a proteomic expression profile with other host\u2010associated facultative intracellular bacteria. In summary, this study describes the global protein expression pattern of <jats:italic>T. whipplei<\/jats:italic> and reveals some specific features of the <jats:italic>T. whipplei<\/jats:italic> proteome.<\/jats:p>","DOI":"10.1002\/pmic.200700889","type":"journal-article","created":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T06:43:21Z","timestamp":1235717001000},"page":"1593-1616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Global proteomic pattern of <b><i>Tropheryma whipplei<\/i><\/b>: A Whipple's disease 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