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Here, we provide a comprehensive study of two types of anoxia, using a proteomics approach to compare changes in protein expression. The two investigated situations were 30\u2005min of chemical anoxia (10\u2005mM NaN<jats:sub>3<\/jats:sub>) followed by reoxygenation overnight (CR) and 2\u2005h of N<jats:sub>2<\/jats:sub>\u2010induced anoxia (achieved by flushing with N<jats:sub>2<\/jats:sub>) followed by reoxygenation overnight (NR), after which samples were resolved by 2\u2010DE. Forty\u2010five protein spots changed their abundance in response to CR and 35 protein spots changed their abundance in response to NR, but only six proteins changed their abundance in response to both stimuli. By the means of MS\/MS, 40 protein spots were identified including proteins involved in processes like cell protection and protein synthesis. It was also revealed that the level of a number of keratins was down\u2010regulated. This study therefore provides a valuable comparison of two different anoxia models and shows that great care should be taken when comparing the effects of anoxia in studies that have used different types and durations of anoxia.<\/jats:p>","DOI":"10.1002\/pmic.200700944","type":"journal-article","created":{"date-parts":[[2008,5,19]],"date-time":"2008-05-19T11:17:35Z","timestamp":1211195855000},"page":"2035-2044","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Comparison of two anoxia models in rainbow trout cells by a 2\u2010DE and MS\/MS\u2010based proteome approach"],"prefix":"10.1002","volume":"8","author":[{"given":"Tune","family":"Wulff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Else K.","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Roepstorff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flemming","family":"Jessen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2005.03.006"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(02)00080-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/08941930500328862"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21062"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.3.H874"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003950170041"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00009.2003"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.2459\/01.JCM.0000199792.32479.ce"},{"key":"e_1_2_1_10_2","first-page":"353","article-title":"Ischaemic preconditioning: from molecular characterisation to clinical application\u2013part I","volume":"62","author":"Riksen N. 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