{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:31:09Z","timestamp":1791307869078,"version":"4.3.1"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Venoms have evolved over millions of years into potent cocktails of bioactive peptides and proteins. These compounds can be of great value to the pharmaceutical industry for numerous clinical applications. In this study, a novel proteomic \u2013 bioinformatic approach was utilised, where chromatography followed by gel electrophoresis was utilised to separate the venom peptides\/proteins of <jats:italic>Heterometrus longimanus (Asian black scorpion).<\/jats:italic> Purified peptides were analysed by tandem mass spectrometry, <jats:italic>de novo<\/jats:italic> sequenced and then homology matched against known peptides in the Swiss\u2010Prot protein database. Numerous potentially biologically active peptide matches were discovered, and a simple scoring system applied to putatively assign functions to the peptides. As a validation of this approach, the functional composition of the experimentally derived proteome is similar to that of other scorpions, and contains a potent mix of toxins, antimicrobials and ionic channel inhibitors.<\/jats:p>","DOI":"10.1002\/pmic.200700948","type":"journal-article","created":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T14:18:07Z","timestamp":1201875487000},"page":"1081-1096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":151,"title":["Proteomic analysis of the venom of <b><i>Heterometrus longimanus<\/i><\/b> (Asian black scorpion)"],"prefix":"10.1002","volume":"8","author":[{"given":"Scott","family":"Bringans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soren","family":"Eriksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulene","family":"Kendrick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Gopalakrishnakone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreja","family":"Livk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Lock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Lipscombe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,3,7]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"579","article-title":"A list of animal toxins and some other natural products with biological activity","volume":"40","author":"Theakston R. 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