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To establish a standard throughput\u2010potential profiling platform for membrane proteins, Triton X\u2010100\u2010solubilized rat liver microsomal proteins were separated on a 2\u2010D separation system (2\u2010D liquid phase fractionation (PF2D)) in two different pH ranges (4.0\u20138.5 and 7.0\u201310.5). This system produced 182 proteins with more than two transmembrane domain (TMD), including 16\u2005TMDs with high confidence. Comparative 2\u2010D liquid maps with high resolution and reproducibility have been constructed for liver microsome from the phenobarbital (PB) treated rats. PF2D was also found to be useful for the semiquantification of some representative cytochrome P450 family proteins (<jats:italic>e.g<\/jats:italic>., cytochrome P450 2B2) that were induced by PB treatment compared with untreated controls. Thus, the combination of both high\u2010detection capacity and rapid preliminary semiquantification in a PF2D platform could become a standard system for the routine analysis of membrane proteins.<\/jats:p>","DOI":"10.1002\/pmic.200701022","type":"journal-article","created":{"date-parts":[[2008,6,4]],"date-time":"2008-06-04T18:29:03Z","timestamp":1212604143000},"page":"2168-2177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Establishment of a PF2D\u2010MS\/MS platform for rapid profiling and semiquantitative analysis of membrane protein biomarkers"],"prefix":"10.1002","volume":"8","author":[{"given":"Hyoung\u2010Joo","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Seok","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Yun","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Ki","family":"Paik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,6,4]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"2002"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560070420"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600156-MCP200"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400943"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400993"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1054::AID-ELPS1054>3.0.CO;2-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"6470","DOI":"10.1016\/S0021-9258(19)84586-6","article-title":"Microsomal enzymes of cholesterol biosynthesis from lanosterol: Solubilization and purification of steroid 8\u2010isomerase","volume":"261","author":"Paik Y. 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