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Extensive researches at DNA level indicated that generation of ROS from the reduction of Cr(VI) leading to DNA damage is the major cause of the toxicity and carcinogenicity of Cr(VI). The present study in cellular and protein levels confirmed that Cr(VI) induced apoptosis of lung epithelial cells (LEC) <jats:italic>via<\/jats:italic> ROS generation. To view the differentially expressed proteins in the process of Cr(VI) reduction, subcellular proteomics was applied and allowed the identification of more than 30 proteins with expression alteration. Most of those proteins are correlated with ROS\u2010elicited responses, which were further validated by Western blotting analysis, induction of p53 pathway and antioxidative treatment. The current findings provided additional evidence in protein level to support the claim that ROS generated during the process of Cr(VI) reduction are involved in the Cr(VI)\u2010induced toxicity and carcinogenesis.<\/jats:p>","DOI":"10.1002\/pmic.200701050","type":"journal-article","created":{"date-parts":[[2008,6,18]],"date-time":"2008-06-18T08:58:41Z","timestamp":1213779521000},"page":"2420-2429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Proteomic analysis of chromium cytotoxicity in cultured rat lung epithelial cells"],"prefix":"10.1002","volume":"8","author":[{"given":"Ting","family":"Lei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Yu","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u2010Sheng","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongwei","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jen\u2010Fu","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,6,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajim.4700170205"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-364931-7.50008-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-002-0343-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/13.7.1159"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/12.9.1733"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(84)90050-7"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/109374099281241"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.99107s15"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx0000116"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00161a049"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/17.7.1511"},{"key":"e_1_2_1_13_2","first-page":"1307","article-title":"Chromium\u2010induced genotoxicity and apoptosis: Relationship to chromium carcinogenesis (review)","volume":"5","author":"Singh J.","year":"1998","journal-title":"Oncol. 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