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Using the purified hepatocytes, we compared the protein profiles by 2\u2010DE and LC\u2010MS between HBV\u2010transgenic (Tg) and corresponding background mice. Twenty\u2010seven altered proteins were identified in hepatocytes from HBV\u2010Tg mice, among which 13 proteins were involved in mitochondrion metabolism pathway including tricarboxylic acid (TCA) cycle and oxidative response; four proteins (SELENBP, SCP2, RGN and PRDX1) were also dramatically changed in liver samples from HBV\u2010infected patients. Important genes (<jats:italic>gpx, sod, ogg<\/jats:italic> et al.) correlated to oxidative damage were up\u2010regulated in the liver of HBV\u2010Tg mice. Reactive oxygen species production was significantly increased while ATP production was decreased in liver mitochondria from HBV\u2010Tg mice. Moreover, hepatocytes of HBV\u2010Tg mice were more sensitive to hydrogen peroxide\u2010induced cell death than that of wild\u2010type control. Using 2\u2010D Western blotting analysis, eight hepatocyte proteins were found to react with sera of HBV\u2010Tg mice but not with that of background mice. Interestingly, two (Etfa and Dmgdh) of the eight reactive proteins were overexpressed in HBV\u2010Tg mice. We believe this study is the first proteomic and seroproteome analysis of HBV\u2010infected mammalian hepatocyte and provides insightful links between HBV infection and HBV\u2010induced liver diseases.<\/jats:p>","DOI":"10.1002\/pmic.200701053","type":"journal-article","created":{"date-parts":[[2008,12,3]],"date-time":"2008-12-03T03:43:57Z","timestamp":1228275837000},"page":"87-105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Hepatocytes proteomic alteration and seroproteome analysis of HBV\u2010transgenic mice"],"prefix":"10.1002","volume":"9","author":[{"given":"Chen","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199712113372406"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2005.00543.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21597"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21872"},{"key":"e_1_2_1_6_2","first-page":"373","article-title":"Hepatitis B virus down\u2010regulates expressions of MHC class I molecules on hepatoplastoma cell line","volume":"3","author":"Chen Y. 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