{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:25:07Z","timestamp":1791307507325,"version":"4.3.1"},"reference-count":48,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T00:00:00Z","timestamp":1216857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Acne is a human disease of the sebaceous hair follicle. Unlike humans, most animals produce little or no triglycerides in hair follicles to harbor\n                    <jats:italic>Propionibacterium acnes<\/jats:italic>\n                    a fact that has encumbered the development of novel treatments for acne lesions. Although genetic mutant mice with acne\u2010like skins have been used for screening anti\u2010acne drugs, the mice generally have deficits in immune system that turns out to be inappropriate to generate antibodies for developing acne vaccines. Here, we employed a bioengineering approach using a tissue chamber integrated with a dermis\u2010based cell\u2010trapped system (DBCTS) to mimic the\n                    <jats:italic>in vivo<\/jats:italic>\n                    microenvironment of acne lesions. Human sebocyte cell lines were grown in DBCTS as a scaffold and inserted into a perforated tissue chamber. After implantation of a tissue chamber bearing human sebocytes into ICR mice,\n                    <jats:italic>P. acnes<\/jats:italic>\n                    or PBS was injected into a tissue chamber to induce host immune response. Infiltrated cells such as neutrophils and macrophages were detectable in tissue chamber fluids. In addition, a proinflammatory cytokine macrophage\u2010inflammatory protein\u20102 (MIP\u20102) was elevated after\n                    <jats:italic>P. acnes<\/jats:italic>\n                    injection. In tissue chamber fluids, 13 proteins including secreted proteins and cell matrix derived from mouse, human cells or\n                    <jats:italic>P. acnes<\/jats:italic>\n                    were identified by proteomics using isotope\u2010coded protein label (ICPL) coupled to nano\u2010LC\u2010MS analysis. After\n                    <jats:italic>P. acnes<\/jats:italic>\n                    infection, four proteins including fibrinogen, \u03b1 polypeptide, fibrinogen \u03b2 chain, S100A9, and serine protease inhibitor A3K showed altered concentrations in the mimicked acne microenvironment. The bioengineered acne model thus provides an\n                    <jats:italic>in vivo<\/jats:italic>\n                    microenvironment to study the interaction of host with\n                    <jats:italic>P. acnes<\/jats:italic>\n                    and offers a unique set\u2010up for screening novel anti\u2010acne drugs and vaccines.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200800044","type":"journal-article","created":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T01:47:19Z","timestamp":1216864039000},"page":"3406-3415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Bioengineering a humanized acne microenvironment model: Proteomics analysis of host responses to\n                    <b>\n                      <i>Propionibacterium acnes<\/i>\n                    <\/b>\n                    infection\n                    <b>\n                      <i>in vivo<\/i>\n                    <\/b>"],"prefix":"10.1002","volume":"8","author":[{"given":"Teruaki","family":"Nakatsuji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Po","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Ru","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Youn","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery W.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos C.","family":"Zouboulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Ming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.03.004"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610-0387.2006.06053.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000065856"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2006.01671.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.41.5.1269-1270.1981"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000096169"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/15569520701502815"},{"key":"e_1_2_1_9_2","first-page":"27","article-title":"Impaired antibody response against T\u2010dependent antigens in rhino mice","volume":"40","author":"Takaoki M.","year":"1980","journal-title":"Immunology"},{"key":"e_1_2_1_10_2","first-page":"531","article-title":"Immunohistochemical studies of the acne\u2010like inflammatory model","volume":"39","author":"Toho M.","year":"1990","journal-title":"Jikken Dobutsu"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/146.4.487"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/376533"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700425"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2003.12.004"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600163"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0520"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400873"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500680"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0704534"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth785"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2006.00803.x"},{"key":"e_1_2_1_22_2","first-page":"9","article-title":"Clinical considerations in the treatment of acne vulgaris and other inflammatory skin disorders: Focus on antibiotic resistance","volume":"79","author":"Leyden J. 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