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Treatment of <jats:italic>B. malayi<\/jats:italic>\u2010infected jirds with tetracycline eliminates <jats:italic>Wolbachia<\/jats:italic>, which affects parasite survival and fitness. In the present study we have tried to identify parasite proteins that are affected when <jats:italic>Wolbachia<\/jats:italic> is targeted by tetracycline. For this <jats:italic>Wolbachia<\/jats:italic> depleted parasites (<jats:italic>B. malayi<\/jats:italic>) were obtained by tetracycline treatment of infected Mongolian jirds (<jats:italic>Meriones unguiculatus<\/jats:italic>) and their protein profile after 2\u2010DE separation was compared with that of untreated parasites harboring <jats:italic>Wolbachia<\/jats:italic>. Approximately 100 protein spots could be visualized followed by CBB staining of 2\u2010D gel and included for comparative analysis. Of these, 54 showed differential expressions, while two new protein spots emerged (of 90.3 and 64.4\u2009kDa). These proteins were subjected to further analysis by MALDI\u2010TOF for their identification using <jats:italic>Brugia<\/jats:italic> coding sequence database composed of both genomic and EST sequences. Our study unravels two crucial findings: (i) the parasite or <jats:italic>Wolbachia<\/jats:italic> proteins, which disappeared\/down\u2010regulated appear be essential for parasite survival and may be used as drug targets and (ii) tetracycline treatment interferes with the regulatory machinery vital for parasites cellular integrity and defense and thus could possibly be a molecular mechanism for the killing of filarial parasite. This is the first proteomic study substantiating the wolbachial genome integrity with its nematode host and providing functional genomic data of human lymphatic filarial parasite <jats:italic>B. malayi<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/pmic.200800324","type":"journal-article","created":{"date-parts":[[2009,8,31]],"date-time":"2009-08-31T06:08:49Z","timestamp":1251698929000},"page":"4192-4208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Tetracycline treatment targeting <i>Wolbachia<\/i> affects expression of an array of proteins in <i>Brugia malayi<\/i> parasite"],"prefix":"10.1002","volume":"9","author":[{"given":"Anil","family":"Dangi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish","family":"Vedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeetendra Kumar","family":"Nag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sameer","family":"Paithankar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahendra Pratap","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santosh Kumar","family":"Kar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuradha","family":"Dube","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailja","family":"Misra\u2010Bhattacharya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,31]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1998.0591"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1998.4383"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.01.006"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(75)90110-8"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(99)01533-1"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/tpbi.1993.1020"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1995.0196"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11154"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(02)00377-6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(00)01812-2"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.8.1429"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(00)01661-6"},{"key":"e_1_2_7_14_2","first-page":"614","article-title":"Wolbachia: potential targets for control of filariasis","volume":"80","author":"Mohanty B. 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