{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T14:59:07Z","timestamp":1773673147788,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T00:00:00Z","timestamp":1231459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The structure of the porin complexes of <jats:italic>Neisseria meningitidis<\/jats:italic> was assessed in the vaccine strain H44\/76 and its homologous mutants lacking the main porins (PorA and PorB) and other outer membrane (OM) components (RmpM and FetA). The analysis using 1\u2010D blue native (BN) electrophoresis, 2\u2010D BN\/SDS\u2010PAGE and 2\u2010D diagonal electrophoresis, followed by LC\/MS\u2010MS (for 1\u2010D gels) or MALDI\u2010TOF (for 2\u2010D gels) revealed at least six porin complexes in the wild\u2010type strain with molecular masses (MW) ranging from 145 to 195\u2005kDa and variable composition: The two higher MW complexes are formed by PorA, PorB and RmpM, the following three are formed by PorA and PorB, and the lower MW one is formed by only PorB. Complexes in the mutants lacking either PorA, PorB or RmpM, but not those in the mutant lacking FetA, were alterered respect to those in the wild\u2010type strain. The most evident alteration was seen in the mutant lacking PorB, in which PorA formed only a high MW complex (\u2248\ufe02800\u2005kDa). Our results suggest that PorA and PorB could form a \u2018basic\u2019 template for the transportation systems in the OM of the meningococci. Other proteins (such as RmpM) could be transiently associated to the porin complexes, depending on the specific tranport needs at different stages of the meningococcal life cycle, resulting in a dynamic net of pores of variable composition.<\/jats:p>","DOI":"10.1002\/pmic.200800486","type":"journal-article","created":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T05:15:40Z","timestamp":1231478140000},"page":"648-656","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Analysis of outer membrane porin complexes of <b><i>Neisseria meningitidis<\/i><\/b> in wild\u2010type and specific knock\u2010out mutant strains"],"prefix":"10.1002","volume":"9","author":[{"given":"Juan","family":"Marzoa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Abel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Feavers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Criado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos M.","family":"Ferreir\u00f3s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)90340-9"},{"key":"e_1_2_1_3_2","volume-title":"Emerging Strategies in the Fight Against Meningitis: Molecular and Cellular Aspects","author":"Ferreir\u00f3s C. 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