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Previous, studies in our laboratory have shown that prior exposure to an IFN\u2010\u03b3\u2010producing\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    strain (H99\u03b3) elicits protective immunity against a second pulmonary\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    challenge. Here, we characterized the antibody response produced in mice protected against experimental pulmonary\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    infection compared to nonprotected mice. Moreover, we evaluated the efficacy of using serum antibody from protected mice to detect immunodominant\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    proteins. Protected mice were shown to produce significantly more\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    \u2010specific antibodies following a second experimental pulmonary cryptococcal challenge compared to nonprotected mice. Immunoblot analysis of\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    proteins resolved by 2\u2010DE using serum from nonprotected mice failed to show any reactivity. In contrast, serum from protected mice was reactive with several cryptococcal protein spots. Analysis of these spots by capillary HPLC\u2010ESI\u2010MS\/MS identified several cryptococcal proteins shown to be associated with the pathogenesis of cryptococcosis. Our studies demonstrate that mice immunized with\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    strain H99\u03b3 produce antibodies that are immune reactive against specific cryptococcal proteins that may provide a basis for the development of immune based therapies that induce protective anticryptococcal immune responses.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200800713","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T05:22:17Z","timestamp":1238649737000},"page":"2578-2588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A proteomic\u2010based approach for the identification of immunodominant\n                    <b>\n                      <i>Cryptococcus neoformans<\/i>\n                    <\/b>\n                    proteins"],"prefix":"10.1002","volume":"9","author":[{"given":"Mattie","family":"Young","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Macias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Floyd L.","family":"Wormley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/CMR.8.4.515","article-title":"Cryptococcosis in the era of AIDS \u2013 100\u2005years after the discovery of Cryptococcus neoformans","volume":"8","author":"Mitchell T. 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