{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:35:43Z","timestamp":1788870943383,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2009,7,28]],"date-time":"2009-07-28T00:00:00Z","timestamp":1248739200000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Research Council for Natural Sciences"},{"name":"Danish Centre for Advanced Food Studies"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cereal seed germination involves a complex coordination between different seed tissues. Plasma membranes must play crucial roles in coordination and execution of germination; however, very little is known about seed plasma membrane proteomes due to limited tissue amounts combined with amphiphilicity and low abundance of membrane proteins. A fraction enriched in plasma membranes was prepared from embryos dissected from 18\u2009h germinated barley seeds using aqueous two\u2010phase partitioning. Reversed\u2010phase chromatography on C\n                    <jats:sub>4<\/jats:sub>\n                    resin performed in micro\u2010spin columns with stepwise elution by 2\u2010propanol was used to reduce soluble protein contamination and enrich for hydrophobic proteins. Sixty\u2010one proteins in 14 SDS\u2010PAGE bands were identified by LC\u2010MS\/MS and database searches. The identifications provide new insight into the plasma membrane functions in seed germination.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200800745","type":"journal-article","created":{"date-parts":[[2009,7,28]],"date-time":"2009-07-28T09:26:36Z","timestamp":1248773196000},"page":"3787-3794","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The plasma membrane proteome of germinating barley embryos"],"prefix":"10.1002","volume":"9","author":[{"given":"Radovan","family":"Hynek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birte","family":"Svensson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole N\u00f8rregaard","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vibeke","family":"Barkholt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Finnie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,28]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2008.12.001"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-5210(03)00033-X"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700766"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0602850"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-924111"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2000.00695.x"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.045708"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2004.09.016"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.84.4.1197"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700002-MCP200"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch209"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.053041"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500574"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2007.09.003"},{"key":"e_1_2_3_16_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1079\/SSR200176","article-title":"Seed lipoxygenases: occurrence and functions","volume":"11","author":"Loiseau J.","year":"2001","journal-title":"Seed Sci. 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