{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:02:08Z","timestamp":1791266528856,"version":"4.1.0"},"reference-count":107,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,6,12]],"date-time":"2009-06-12T00:00:00Z","timestamp":1244764800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006488","name":"Institut National de la Recherche Agronomique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006488","id-type":"DOI","asserted-by":"crossref"}]},{"name":"French Minist\u00e8re de l'agriculture et de la p\u00eache","award":["DGAL N\u00b0 A03\/02"],"award-info":[{"award-number":["DGAL N\u00b0 A03\/02"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While <jats:italic>Listeria monocytogenes<\/jats:italic> is responsible for listeriosis, it is also a saprophytic species with exceptional survival aptitudes. Secreted proteins are one of the main tools used by bacteria to interact with their environment. In order to take into account the biodiversity of <jats:italic>L. monocytogenes<\/jats:italic> species, exoproteomic analysis was carried out on 12 representative strains. Following 2\u2010DE and MALDI\u2010TOF MS, a total of 151 spots were identified and corresponded to 60 non\u2010orthologous proteins. To categorize and analyze these proteomic data, a rational bioinformatic approach predicting final subcellular localization was carried out. Fifty\u2010two out of the 60 proteins identified (86.7%) were indeed predicted as localized in the extracellular milieu (gene ontology (GO): 0005576). Most of them (65.4%) were actually predicted as secreted <jats:italic>via<\/jats:italic> the Sec translocon. Comparative analysis allowed for proteins found in all or only in a subset of <jats:italic>L. monocytogenes<\/jats:italic> strains to be defined. While the core exoproteome included most proteins related to bacterial virulence, cell wall biogenesis, as well as proteins secreted by unknown pathways, a slight variation in the protein members of these categories were observed and constituted the variant exoproteome. This investigation resulted in the first definition of the core and variant exoproteomes of <jats:italic>L. monocytogenes<\/jats:italic> where corollaries on bacterial physiology are further discussed.<\/jats:p>","DOI":"10.1002\/pmic.200800765","type":"journal-article","created":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T14:11:01Z","timestamp":1245075061000},"page":"3136-3155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Insight into the core and variant exoproteomes of Listeria monocytogenes species by comparative subproteomic analysis"],"prefix":"10.1002","volume":"9","author":[{"given":"Emilie","family":"Dumas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micka\u00ebl","family":"Desvaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Chambon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"H\u00e9braud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,12]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"23","article-title":"Nursing case management in the NICU: enhanced coordination for discharge planning","volume":"17","author":"Forsyth T. J.","year":"1998","journal-title":"Neonatal Netw."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46495-0"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.2.169"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090934"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/.61.2.136-169.1997"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00035.x"},{"key":"e_1_2_6_8_2","first-page":"1235","volume-title":"Bergey's Manual of Systematic Bacteriology","author":"Seeliger H. P. R.","year":"1986"},{"key":"e_1_2_6_9_2","volume-title":"Micro\u2010organisms in Foods: Microbiological Specifications of Food Pathogens","author":"ICMSF (International Commission on Microbiological Specifications for Foods of the International Union of Biological Societies)","year":"1996"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.728-737.2002"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7336-7342.2003"},{"key":"e_1_2_6_12_2","first-page":"21","volume-title":"Listeria, Listeriosis, and Food Safety","author":"Fenlon D. R.","year":"1999"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01609.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3429-3436.2003"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00460-3"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.2505-2512.2006"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/35073038"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400936"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600858"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401024"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6420"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02730-06"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1855"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420051414.ch12"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.11.4683"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(85)90051-6"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.03.016"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1273\/cbij.4.142"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(02)00289-9"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti057"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh378"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.08.140"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-167"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03090.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl124"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-58"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.4.641"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0303703"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg360"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.6026\/97320630001176"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-7-2065"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.22"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(96)80056-X"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl841"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01019-6"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.5857"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1043"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80134-2"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl910"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh097"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj063"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03023-05"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj161"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.9.849"},{"key":"e_1_2_6_57_2","first-page":"175","article-title":"A hidden Markov model for predicting transmembrane helices in protein sequences","volume":"6","author":"Sonnhammer E. L.","year":"1998","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.4.378"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0305103"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl677"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.721"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.02.025"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21018"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.182801"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200402080"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00008-06"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.70.1.192-221.2006"},{"key":"e_1_2_6_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.9.2910-2919.2003"},{"key":"e_1_2_6_69_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211055200"},{"key":"e_1_2_6_70_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01652.x"},{"key":"e_1_2_6_71_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4621825.x"},{"key":"e_1_2_6_72_2","first-page":"1200","article-title":"Mapping of carbon flow distribution in the central metabolic pathways of Clostridium \n                  cellulolyticum: Direct comparison of bacterial metabolism with a soluble versus an insoluble carbon source","volume":"14","author":"Desvaux M.","year":"2004","journal-title":"J. Microbiol. Biotechnol."},{"key":"e_1_2_6_73_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-126"},{"key":"e_1_2_6_74_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00773-06"},{"key":"e_1_2_6_75_2","first-page":"29","article-title":"Analysis of the peptidoglycan hydrolases of Listeria \n                  monocytogenes: multiple enzymes with multiple functions","volume":"53","author":"Popowska M.","year":"2004","journal-title":"Pol. J. Microbiol."},{"key":"e_1_2_6_76_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-2-r11"},{"key":"e_1_2_6_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00976-06"},{"key":"e_1_2_6_78_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh036"},{"key":"e_1_2_6_79_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447"},{"key":"e_1_2_6_80_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3233\/ISB-00007","article-title":"Sources of systematic error in functional annotation of genomes: domain rearrangement, non\u2010orthologous gene displacement and operon disruption","volume":"1","author":"Galperin M. Y.","year":"1998","journal-title":"In Silico Biol."},{"key":"e_1_2_6_81_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205009"},{"key":"e_1_2_6_82_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05252.x"},{"key":"e_1_2_6_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.1.131-139.1999"},{"key":"e_1_2_6_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.556-568.2006"},{"key":"e_1_2_6_85_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.003"},{"key":"e_1_2_6_86_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606249200"},{"key":"e_1_2_6_87_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03072.x"},{"key":"e_1_2_6_88_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-006-0122-8"},{"key":"e_1_2_6_89_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.24.8385-8394.2005"},{"key":"e_1_2_6_90_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03425.x"},{"key":"e_1_2_6_91_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm954"},{"key":"e_1_2_6_92_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2900205"},{"key":"e_1_2_6_93_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02701-07"},{"key":"e_1_2_6_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.11.003"},{"key":"e_1_2_6_95_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-7612-5_10"},{"key":"e_1_2_6_96_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608279103"},{"key":"e_1_2_6_97_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400928"},{"key":"e_1_2_6_98_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500091"},{"key":"e_1_2_6_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.2.207-233.2004"},{"key":"e_1_2_6_100_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00683.x"},{"key":"e_1_2_6_101_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04138.x"},{"key":"e_1_2_6_102_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500879102"},{"key":"e_1_2_6_103_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2007017"},{"key":"e_1_2_6_104_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.10.6039-6048.2005"},{"key":"e_1_2_6_105_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/154.10.944"},{"key":"e_1_2_6_106_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.120.1.105"},{"key":"e_1_2_6_107_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1217-1224.2003"},{"key":"e_1_2_6_108_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0399-077X(05)81348-4"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200800765","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200800765","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200800765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:24:35Z","timestamp":1761063875000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200800765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":107,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1002\/pmic.200800765"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200800765","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]},"assertion":[{"value":"2008-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}