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The objective of the present study was to establish a comprehensive profile of the abundant proteins of the pig conceptus at the time prior to implantation and identify stage\u2010specific changes during elongation. The abundant proteins of a homogenous population of gestational day\u201011 ovoid (0.7\u20131\u2005cm) and gestational day\u201012 filamentous (15\u201320\u2005cm) porcine concepti were compared by extracting proteins from three independent conceptus pools and separating the proteins by 2\u2010DE. Proteins in 305 spots were analyzed by MALDI\u2010TOF or additionally by LC\u2010MS\/MS and 275 were positively identified representing 174 distinct proteins. The proteins could be classified into the following functional categories: cell proliferation\/differentiation, cytoskeleton, metabolism, and stress response. Based on spot density, 35 proteins associated with cell proliferation, differentiation, apoptosis, and embryo\/maternal signaling, were found to be differentially expressed between ovoid and filamentous concepti. A comparison of the protein expression profile with transcriptomic data from pig concepti of the same developmental stages identified similarities and dissimilarities between protein and mRNA expression profiles. This proteomic study helps to elucidate the biological mechanisms underlying the early embryonic development of the pig.<\/jats:p>","DOI":"10.1002\/pmic.200800776","type":"journal-article","created":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T02:23:25Z","timestamp":1240539805000},"page":"2678-2694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Comparative proteomic and regulatory network analyses of the elongating pig conceptus"],"prefix":"10.1002","volume":"9","author":[{"given":"S\u00e9verine A.","family":"Degrelle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Ann","family":"Blomberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wesley M.","family":"Garrett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil C.","family":"Talbot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,5,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091900112"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.4.941"},{"key":"e_1_2_1_4_2","first-page":"261","article-title":"Embryonic and uterine development during early pregnancy in pigs","volume":"40","author":"Stroband H. 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