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For the recognition of disease\u2010associated changes in bovine CSF protein patterns, a detailed knowledge of this proteome is a prerequisite. The absence of a high\u2010resolution CSF proteome map prompted us to determine all bovine CSF protein spots that can be visualised on 2\u2010D protein gels. Using state\u2010of\u2010the\u2010art 2\u2010DE technology for proteome mapping of bovine <jats:italic>ante mortem<\/jats:italic> CSF combined with sensitive fluorescent protein staining and MALDI\u2010TOF\/TOF MS for protein identification, a highly detailed 2\u2010DE map of the bovine CSF proteome was established. 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