{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:41:22Z","timestamp":1790649682003,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,6,27]],"date-time":"2009-06-27T00:00:00Z","timestamp":1246060800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"crossref","award":["RG\/06\/004\/21625"],"award-info":[{"award-number":["RG\/06\/004\/21625"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"crossref","award":["04\/RP1\/B499"],"award-info":[{"award-number":["04\/RP1\/B499"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent studies have suggested a protective role of hsp27 against atherosclerosis and transplant graft vasculopathy. Here we have investigated the effects of over\u2010expression of wild\u2010type hsp27 and its phosphorylation mimics on proliferation of human endothelial cells (ECs) and smooth muscle cells (SMCs). ECs and SMCs cultured from human blood vessels or cells lines (human microvascular endothelial cell line and human telomerase reverse transcriptase subunit SMC) were infected with adenovirus containing DNA from wild\u2010type hsp27, hyper\u2010phosphorylated hsp27 mimic (3D hsp27), hypo\u2010phosphorylated hsp27 mimic (3A hsp27) or anti\u2010sense hsp27, and proliferation measured over the next 5 days. Protein extracts from infected cells were subjected to proteomic analysis using 2\u2010D DIGE. Over\u2010expression of 3D hsp27 and anti\u2010sense hsp27 but not 3A hsp27 mimic caused significant inhibition of proliferation of ECs and SMCs. Proteomic analysis focussed on proteins that were significantly down\u2010regulated by the 3D hsp27 mutant. The cell cycling proteins stathmin, cofilin and ubiquitination enzymes fullfilled these criteria. 1\u2010D Western blots of infected human microvascular endothelial cell line and human telomerase reverse transcriptase subunit SMC confirmed down\u2010regulation of stathmin, cofilin and ubiquitination enzymes by 3D hsp27. The phosphorylation status of hsp27 is an important regulator of proliferation of human vascular ECs and SMCs; possibly contributing to cardiovascular protection.<\/jats:p>","DOI":"10.1002\/pmic.200800961","type":"journal-article","created":{"date-parts":[[2009,6,27]],"date-time":"2009-06-27T03:31:41Z","timestamp":1246073501000},"page":"3383-3394","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Effect of phosphorylated hsp27 on proliferation of human endothelial and smooth muscle cells"],"prefix":"10.1002","volume":"9","author":[{"given":"Deborah","family":"Trott","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciara A.","family":"McManus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody L.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryony","family":"Brennan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene L.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.2.425"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-004-0483-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000148825.99184.50"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000174815.10996.08"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000136814.87170.B1"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.541219"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.172155"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021601103096"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1379\/1466-1268(2002)007<0269:HSPPHB>2.0.CO;2"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1379\/1466-1268(2002)007<0167:ACASHS>2.0.CO;2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199701)170:1<19::AID-JCP3>3.0.CO;2-O"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.2174\/138161207780831257"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000178991.71605.18"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000246086.93555.f3"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.embor847"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12613748"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.96.12.4343"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610987200"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500083"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501223"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150090108"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200011)21:17<3666::AID-ELPS3666>3.0.CO;2-6"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)48354-8"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06425.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00453.2006"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.5.909"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00914.x"},{"key":"e_1_2_6_29_2","first-page":"6405","article-title":"Angiogenesis inhibitors target the endothelial cell cytoskeleton through altered regulation of heat shock protein 27 and cofilin","volume":"63","author":"Keezer S. 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