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We present the first comprehensive proteome analysis of this pathogen, profiling proteins from bacteria cultured\n                    <jats:italic>in vitro<\/jats:italic>\n                    and bacterial isolates from the large bowel of infected gnotobiotic piglets (\n                    <jats:italic>in vivo<\/jats:italic>\n                    ). Overall, 1061 distinct gene products were identified. Differential display analysis revealed that SD1 cells switched to an anaerobic energy metabolism\n                    <jats:italic>in vivo<\/jats:italic>\n                    . High\n                    <jats:italic>in vivo<\/jats:italic>\n                    abundances of amino acid decarboxylases (GadB and AdiA) which enhance pH homeostasis in the cytoplasm and protein disaggregation chaperones (HdeA, HdeB and ClpB) were indicative of a coordinated bacterial survival response to acid stress. Several type III secretion system effectors were increased in abundance\n                    <jats:italic>in vivo<\/jats:italic>\n                    , including OspF, IpaC and IpaD. These proteins are implicated in invasion of colonocytes and subversion of the host immune response in\n                    <jats:italic>S. flexneri<\/jats:italic>\n                    . These observations likely reflect an adaptive response of SD1 to the hostile host environment. Seven proteins, among them the type III secretion system effectors OspC2 and IpaB, were detected as antigens in Western blots using piglet antisera. The outer membrane protein OmpA, the heat shock protein HtpG and OspC2 represent novel SD1 subunit vaccine candidates and drug targets.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200900196","type":"journal-article","created":{"date-parts":[[2009,10,7]],"date-time":"2009-10-07T11:16:18Z","timestamp":1254914178000},"page":"5029-5045","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The\n                    <i>Shigella dysenteriae<\/i>\n                    serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins"],"prefix":"10.1002","volume":"9","author":[{"given":"Rembert","family":"Pieper","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanshun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashanth P.","family":"Parmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Ting","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Alami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Donohue\u2010Rolfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Fleischmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott N.","family":"Peterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul","family":"Tzipori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,17]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"World Health Organization Diarrhoeal diseases(update February 2009)2009."},{"key":"e_1_2_6_3_2","first-page":"133","article-title":"Shigellosis","volume":"43","author":"Niyogi S. 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