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Both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (CCA). To identify parasite proteins critical for liver fluke survival and the etiology of CCA, OFFGEL electrophoresis and multiple reaction monitoring were employed to characterize 300 parasite proteins from the <jats:italic>O. viverrini<\/jats:italic> excretory\/secretory products and, utilizing selective labeling and sequential solubilization, from the host\u2010exposed tegument. The excretory\/secretory included a complex mixture of proteins that have been associated with cancers, including proteases of different mechanistic classes and orthologues of mammalian growth factors and anti\u2010apoptotic proteins. Also identified was a cysteine protease inhibitor which, in other helminth pathogens, induces nitric oxide production by macrophages, and, hence may contribute to malignant transformation of inflamed cells. More than 160 tegumental proteins were identified using sequential solubilization of isolated teguments, and a subset of these was localized to the surface membrane of the tegument by labeling living flukes with biotin and confirming surface localization with fluorescence microscopy. These included annexins, which are potential immuno\u2010modulators, and orthologues of the schistosomiasis vaccine antigens Sm29 and tetraspanin\u20102. Novel roles in pathogenesis were suggested for the tegument\u2013host interface since more than ten surface proteins had no homologues in the public databases. The <jats:italic>O. viverrini<\/jats:italic> proteins identified here provide an extensive catalogue of novel leads for research on the pathogenesis of opisthorchiasis and the development of novel interventions for this disease and CCA, as well as providing a scaffold for sequencing the genome of this fluke.<\/jats:p>","DOI":"10.1002\/pmic.200900393","type":"journal-article","created":{"date-parts":[[2010,1,4]],"date-time":"2010-01-04T11:06:00Z","timestamp":1262603160000},"page":"1063-1078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":136,"title":["The secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke <i>Opisthorchis viverrini<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Jason","family":"Mulvenna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banchob","family":"Sripa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brindley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Gorman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm K.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Colgrave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alun","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujeevi","family":"Nawaratna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thewarach","family":"Laha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutas","family":"Suttiprapa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Smout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Loukas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,2,26]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1167","article-title":"Opisthorchis viverrini infection: pathogenesis and clinical features","volume":"34","author":"Harinasuta T.","year":"1984","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1992.tb00928.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(94)90009-4"},{"key":"e_1_2_6_5_2","first-page":"4634","article-title":"Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini\u2010infected syrian golden hamsters","volume":"38","author":"Thamavit W.","year":"1978","journal-title":"Cancer Res."},{"key":"e_1_2_6_6_2","first-page":"313","article-title":"Liver flukes and liver cancer","volume":"33","author":"Vatanasapt V.","year":"1999","journal-title":"Cancer Surv."},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040201"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2004.01234.x"},{"key":"e_1_2_6_9_2","first-page":"1121","article-title":"Liver carcinoma and liver fluke infection","volume":"34","author":"Kim Y. 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