{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T01:11:26Z","timestamp":1789780286097,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2009,10,15]],"date-time":"2009-10-15T00:00:00Z","timestamp":1255564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The pathogenicity of\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    is related to its ability of invading and multiplying in eukaryotic cells. Its main virulence factors are now well characterized, but limited proteomic data is available concerning its adaptation to the intracellular environment. In this study,\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>monocytogenes<\/jats:italic>\n                    EGD (serotype 1\/2a) grown in human THP\u20101 monocytes (24\u2009h) were successfully separated from host organelles and cytosolic proteins by differential and isopycnic centrifugation. For control, we used cell homogenates spiked with bacteria grown in broth. Proteomes from both forms of bacteria were compared using a 2\u2010D\u2010DIGE approach followed by MALDI\u2010TOF analysis to identify proteins. From 1684 distinct spots, 448 were identified corresponding to 245 distinct proteins with no apparent contamination of host proteins. Amongst them, 61 show underexpression (stress defense; transport systems, carbon metabolism, pyrimidines synthesis,\n                    <jats:sc>D<\/jats:sc>\n                    \u2010Ala\u2010\n                    <jats:sc>D<\/jats:sc>\n                    \u2010Ala ligase) and 22 an overexpression (enzymes involved in the synthesis of cell envelope lipids, glyceraldehyde\u20103\u2010phosphate, pyruvate and fatty acids). Our proteomic analysis of intracellular\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>monocytogenes<\/jats:italic>\n                    (i) suggests that bacteria thrive in a more favorable environment than extracellularly, (ii) supports the concept of metabolic adaptation of bacteria to intracellular environment and (iii) may be at the basis of improved anti\u2010\n                    <jats:italic>Listeria<\/jats:italic>\n                    therapy.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200900503","type":"journal-article","created":{"date-parts":[[2009,10,15]],"date-time":"2009-10-15T09:43:41Z","timestamp":1255599821000},"page":"5484-5496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Isolation and 2\u2010D\u2010DIGE proteomic analysis of intracellular and extracellular forms of\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"9","author":[{"given":"S\u00e9bastien","family":"Van de Velde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard","family":"Delaive","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Dieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Carryn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Van Bambeke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Devreese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Raes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Tulkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.1.1"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.081377"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090124"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.004"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.07.043"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1413"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06337.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.006"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.9.3756-3760.1993"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5622-5629.2004"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012363899"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.7174-7180.1997"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00453.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.50.3.314-352.1986"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05956.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2112"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01636-08"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.7069-7077.2000"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1323-1338.2006"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.556-568.2006"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800026"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800266"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1128\/AAC.40.5.1225","article-title":"Effect of recombinant human gamma interferon on intracellular activities of antibiotics against Listeria \n                  monocytogenes in the human macrophage cell line THP\u20101","volume":"40","author":"Scorneaux B.","year":"1996","journal-title":"Antimicrob. 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