{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:40:54Z","timestamp":1778636454758,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hong Kong Research Grants Council","award":["HKU 7624\/06M"],"award-info":[{"award-number":["HKU 7624\/06M"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Candida glabrata<\/jats:italic> is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free\u2010floating or planktonic mode of growth. Therefore, in the present study, we used 2\u2010D DIGE to evaluate the differential proteomic profiles of <jats:italic>C. glabrata<\/jats:italic> under planktonic and biofilm modes of growth. <jats:italic>Candida glabrata<\/jats:italic> biofilms were developed on polystyrene surfaces and age\u2010matched planktonic cultures were obtained in parallel. Initially, biofilm architecture, viability, and antifungal susceptibility were evaluated. Differentially expressed proteins more than 1.5\u2010fold in DIGE analysis were subjected to MS\/MS. The transcriptomic regulation of these biomarkers was evaluated by quantitative real\u2010time PCR. <jats:italic>Candida glabrata<\/jats:italic> biofilms were highly resistant to the antifungals and biocides compared with the planktonic mode of growth. <jats:italic>Candida glabrata<\/jats:italic> biofilm proteome when compared with its planktonic proteome showed upregulation of stress response proteins, while glycolysis enzymes were downregulated. Similar trend could be observed at transcriptomic level. In conclusion, <jats:italic>C. glabrata<\/jats:italic> biofilms possess higher amount of stress response proteins, which may potentially contribute to the higher antifungal resistance seen in <jats:italic>C. glabrata<\/jats:italic> biofilms.<\/jats:p>","DOI":"10.1002\/pmic.200900611","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T08:25:16Z","timestamp":1265012716000},"page":"1444-1454","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Proteomics of drug resistance in <i>Candida glabrata<\/i> biofilms"],"prefix":"10.1002","volume":"10","author":[{"given":"C. Jayampath","family":"Seneviratne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshman P.","family":"Samaranayake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2002.0999"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3268-3272.2002"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/342300"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600304"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.003431"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5409.1837"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02439-06"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.11.3499-3505.2002"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.01.009"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00131-07"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700845"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401064"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701097"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1981.tb01073.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.2961-2967.2003"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00541-08"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014932302518"},{"key":"e_1_2_6_19_2","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts","author":"National Committee for Clinical Laboratory Standards","year":"2002"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2475-2479.2001"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-005-0167-2"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0249"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200011)21:17<3666::AID-ELPS3666>3.0.CO;2-6"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500840"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45762-0"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.08.002"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01473-07"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.4.633-638.2005"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.06.020"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00761.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800031101"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700594"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00161-06"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600332"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.5.1018-1024.2003"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2006.02.037"},{"key":"e_1_2_6_38_2","first-page":"207","article-title":"Comparative proteomic analyses of the yeast Saccharomyces cerevisiae KNU5377 strain against menadione\u2010induced oxidative stress","volume":"17","author":"Kim I.","year":"2007","journal-title":"J. Microbiol. Biotechnol."},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47038-X"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03216.x"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27017-0"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.6831-6837.2005"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.8.3064-3079.2004"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(199907)15:10A<829::AID-YEA423>3.0.CO;2-9"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29040-0"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006163-0"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00684-06"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28745-0"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.04.001"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28616-0"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.07.018"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/010405-0"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900611","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900611","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200900611","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:41:19Z","timestamp":1761043279000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200900611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":51,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1002\/pmic.200900611"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200900611","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]},"assertion":[{"value":"2009-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}