{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T21:08:11Z","timestamp":1770498491947,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,3,17]],"date-time":"2011-03-17T00:00:00Z","timestamp":1300320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Swedish National Science Council"},{"name":"SSF Strategic Center for Translational Cancer Research"},{"name":"Bioinvent"},{"name":"Crafoord Research Foundation"},{"DOI":"10.13039\/501100003461","name":"Gunnar Nilsson Cancer Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003461","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Magnus Bergwall Foundation"},{"DOI":"10.13039\/501100004063","name":"Knut and Alice Wallenberg Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007308","name":"Swedish Medical Association","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007308","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007435","name":"\u00c5ke Wiberg Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007435","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non\u2010fractionated membrane proteomes under non\u2010denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water\u2010soluble proteins.<\/jats:p>","DOI":"10.1002\/pmic.200900808","type":"journal-article","created":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T02:12:33Z","timestamp":1299118353000},"page":"1550-1554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts"],"prefix":"10.1002","volume":"11","author":[{"given":"Linda","family":"Dexlin\u2010Mellby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sandstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linn","family":"Antberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny","family":"Gunnarsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan R.","family":"Hansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl A.K.","family":"Borrebaeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christer","family":"Wingren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,3,17]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800597"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0303-262"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.7.5.673"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2006"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070257x"},{"key":"e_1_2_3_7_2","first-page":"4483","article-title":"Immunophenotyping of leukemias using a cluster of differentiation antibody microarray","volume":"61","author":"Belov L.","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1633513100"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000170"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070204f"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700025"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.01.027"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/15216540500167237"},{"key":"e_1_2_3_14_2","first-page":"458748","article-title":"Differential proteome analysis of the preeclamptic placenta using optimized protein extraction","author":"Centlow M.","year":"2010","journal-title":"J. Biomed. Biotechnol."}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900808","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200900808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:19:38Z","timestamp":1761041978000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200900808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,17]]},"references-count":13,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1002\/pmic.200900808"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200900808","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,17]]},"assertion":[{"value":"2009-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-03-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}