{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:51:20Z","timestamp":1788871880477,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,15]],"date-time":"2010-12-15T00:00:00Z","timestamp":1292371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["20090065565"],"award-info":[{"award-number":["20090065565"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003693","name":"Korea Institute of Science and Technology","doi-asserted-by":"crossref","award":["2E21520"],"award-info":[{"award-number":["2E21520"]}],"id":[{"id":"10.13039\/501100003693","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is well known that the two chemical compounds endothelin\u20101 (ET\u20101) and isoproterenol (ISO) can individually induce cardiac hypertrophy through G protein\u2010coupled receptors in cardiomyocytes. However, the cardiac hypertrophy signaling pathway activated by ET\u20101 and ISO is not well defined. Therefore, we investigated the protein expression profile and signaling transduction in HL\u2010l cardiomyocyte cells treated with ET\u20101 and ISO. Following separation of the cell lysates by using 2\u2010DE and silver staining, we identified 16 protein spots that were differentially expressed as compared to the controls. Of these 16 spots, three changed only after treatment with ET\u20101, whereas four changed only after treatment with ISO, suggesting that these two stimuli could induce different signaling pathways. In order to reveal the differences between ET\u20101\u2010 and ISO\u2010induced signaling, we studied the different events that occur at each step of the signaling pathways, when selected biocomponents were blocked by inhibitors. Our results indicated that ET\u20101 and ISO used different pathways for phosphorylation of glycogen synthase kinase\u20103\u03b2 (GSK3\u03b2). ET\u20101 mainly used the mitogen\u2010activated protein kinase and phosphatidylinositol\u20103\u2010kinase\/AKT pathways to activate GSK3\u03b2, whereas under ISO stimulation, only the phosphatidylinositol\u20103\u2010kinase\/AKT pathway was required to trigger the GSK3\u03b2 pathway. Furthermore, the strength of the GSK3\u03b2 signal in ISO\u2010induced cardiac hypertrophy was stronger than that in ET\u20101\u2010induced cardiac hypertrophy. We found that these two agonists brought about different changes in the protein expression of HL\u20101 cardiomyocytes through distinct signaling pathways even though the destination of the two signaling pathways was the same.<\/jats:p>","DOI":"10.1002\/pmic.201000018","type":"journal-article","created":{"date-parts":[[2010,11,30]],"date-time":"2010-11-30T06:15:23Z","timestamp":1291097723000},"page":"283-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Endothelin\u20101\u2010 and isoproterenol\u2010induced differential protein expression and signaling pathway in HL\u20101 cardiomyocytes"],"prefix":"10.1002","volume":"11","author":[{"given":"Hye\u2010Min","family":"Hong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Joo","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eulsik","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Humayun","family":"Kabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheolju","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Sook","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.206920"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.08.002"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.391"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvp208"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2003.03873.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.05.006"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050275"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142243"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm078"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.02.005"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.20.15239"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1983"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2000.1135"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.04.003"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00986.2003"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.2979"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201007200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060326s"},{"key":"e_1_2_6_20_2","first-page":"113","article-title":"Troubleshooting image analysis in 2DE","volume":"519","author":"Lev\u00e4nen B.","year":"2009","journal-title":"Methods Mol. 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