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Here we report the construction of a protein microarray containing 1901 <jats:italic>C. burnetii<\/jats:italic> ORFs (84% of the entire proteome). This array was probed with Q\u2010fever patient sera and na\u00efve controls in order to discover <jats:italic>C. burnetii<\/jats:italic>\u2010specific seroreactive antigens. Among the 21 seroreactive antigens identified, 13 were significantly more reactive in Q\u2010fever cases than na\u00efve controls. The remaining eight antigens were cross\u2010reactive in both <jats:italic>C. burnetii<\/jats:italic> infected and na\u00efve patient sera. An additional 64 antigens displayed variable seroreactivity in Q\u2010fever patients, and underscore the diversity of the humoral immune response to <jats:italic>C. burnetii<\/jats:italic>. Nine of the differentially reactive antigens were validated on an alternative immunostrip platform, demonstrating proof\u2010of\u2010concept development of a consistent, safe, and inexpensive diagnostic assay alternative. Furthermore, we report here the identification of several new diagnostic antigens and potential subunit vaccine candidates for the highly infectious category B alphaproteobacteria, <jats:italic>C. burnetii<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/pmic.201000064","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T08:55:25Z","timestamp":1271148925000},"page":"2259-2269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Genome\u2010wide profiling of humoral immune response to <i>Coxiella burnetii<\/i> infection by protein microarray"],"prefix":"10.1002","volume":"10","author":[{"given":"Adam","family":"Vigil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocio","family":"Ortega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rie","family":"Nakajima\u2010Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jozelyn","family":"Pablo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas M.","family":"Molina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Chung","family":"Chao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua\u2010Wei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Mei","family":"Ching","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip L.","family":"Felgner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/9.5.935"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/spid.2002.127199"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02365-05"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.159.2.167"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/517453"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb06735.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(94)90325-5"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00146378"},{"key":"e_1_2_6_11_2","first-page":"254","article-title":"Coxiella burnetii antigens for detection of Q fever antibodies by ELISA in human sera","volume":"31","author":"Kovacova E.","year":"1987","journal-title":"Acta Virol."},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02114898"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb42216.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/jcm.30.9.2462-2466.1992","article-title":"Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction","volume":"30","author":"Stein A.","year":"1992","journal-title":"J. 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