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This study focuses on the effects of butenolide, an effective larval settlement inhibitor, on larval settlement at the proteome level in the bryozoan <jats:italic>Bugula neritina<\/jats:italic>. Liquid\u2010phase IEF sample prefractionation combined with 2\u2010DE and MALDI\u2010TOF MS was used to identify the differentially expressed proteins. Substantial changes occurred both in protein abundance and in phosphorylation status during larval settlement and when settling larvae were challenged with butenolide. The proteins that responded to treatment were identified as structural proteins, molecular chaperones, mitochondrial peptidases and calcium\u2010binding proteins. Compared with our earlier results, both genistein and butenolide inhibited larval settlement of <jats:italic>B. neritina<\/jats:italic> primarily by changes in protein abundance and the phosphorylation status of proteins but have different protein targets in the same species. Clearly, to design potent antifouling compounds and to understand the mode of action of compounds, more studies on the effects of different compounds on proteome and phosphoproteome of different larval species are required.<\/jats:p>","DOI":"10.1002\/pmic.201000199","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T13:19:06Z","timestamp":1280323146000},"page":"3435-3446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Changes in the proteome and phosphoproteome expression in the bryozoan <i>Bugula neritina<\/i> larvae in response to the antifouling agent butenolide"],"prefix":"10.1002","volume":"10","author":[{"given":"Pei\u2010Yuan","family":"Qian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue Him","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(00)00150-4"},{"key":"e_1_2_6_3_2","first-page":"849","article-title":"Settlement inhibition of bryozoan larvae by bacterial films and aqueous leachates","volume":"61","author":"Bryan J. 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