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In this study, we investigated the protein composition of the culture filtrate proteins of highly virulent <jats:italic>F. tularensis<\/jats:italic> subsp. <jats:italic>tularensis<\/jats:italic>, strain SCHU S4 and attenuated <jats:italic>F. tularensis<\/jats:italic> subsp. <jats:italic>holarctica<\/jats:italic>, live vaccine strain using a comparative proteomic analysis. The majority of proteins identified in this study have been implicated in virulence mechanisms of other pathogens, and several have been categorized as having moonlighting properties; those that have more than one unrelated function. This profiling study of secreted proteins resulted in the unique detection of acid phosphatase (precursor) A (AcpA), \u03b2\u2010lactamase, and hypothetical protein FTT0484 in the highly virulent strain SCHU S4 secretome. The release of AcpA may be of importance for <jats:italic>F. tularensis<\/jats:italic> subsp. <jats:italic>tularensis<\/jats:italic> virulence due to the recently described AcpA role in the <jats:italic>F. tularensis<\/jats:italic> escape from phagosomes.<\/jats:p>","DOI":"10.1002\/pmic.201000248","type":"journal-article","created":{"date-parts":[[2010,10,15]],"date-time":"2010-10-15T04:31:31Z","timestamp":1287117091000},"page":"4501-4511","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Comparative proteomic profiling of culture filtrate proteins of less and highly virulent <i>Francisella tularensis<\/i> 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