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Lipopolysaccharide (LPS)\u2010induced cytokines and pro\u2010inflammatory protein expression are reduced by minocycline in cultured macrophages. Here, we tested a range of clinically important tetracycline compounds (oxytetracycline, doxycycline, minocycline and tigecycline) and showed that they all inhibited LPS\u2010induced nitric oxide production. We made the novel finding that tigecycline inhibited LPS\u2010induced nitric oxide production to a greater extent than the other tetracycline compounds tested. To identify potential targets for minocycline, we assessed alterations in the macrophage proteome induced by LPS in the presence or absence of a minocycline pre\u2010treatment using 2\u2010DE and nanoLC\u2010MS. We found a number of proteins, mainly involved in cellular metabolism (ATP synthase \u03b2\u2010subunit and aldose reductase) or stress response (heat shock proteins), which were altered in expression in response to LPS, some of which were restored, at least in part, by minocycline. This is the first study to document proteomic changes induced by minocycline. The observation that minocycline inhibits some, but not all, of the LPS\u2010induced proteomic changes shows that minocycline specifically affects some signalling pathways and does not completely inhibit macrophage activation.<\/jats:p>","DOI":"10.1002\/pmic.201000273","type":"journal-article","created":{"date-parts":[[2010,11,25]],"date-time":"2010-11-25T08:13:42Z","timestamp":1290672822000},"page":"42-51","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Proteomic analysis of the anti\u2010inflammatory action of minocycline"],"prefix":"10.1002","volume":"11","author":[{"given":"Christopher R.","family":"Dunston","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen R.","family":"Griffiths","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Staddon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann B.","family":"Vernallis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03219.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00050.2004"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-200311000-00001"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13496"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15769"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.14014"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-122-2-199501150-00001"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070515"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2008.06.028"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251341998"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/1352458506070319"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70270-3"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/417074a"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00046-6"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20335"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1999.00747.x"},{"key":"e_1_2_6_18_2","first-page":"39","article-title":"Differential regulation of MMP\u201013 by chemical modified tetracyclines in osteoblasts","volume":"6","author":"Kirkwood K.","year":"2004","journal-title":"J. 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