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However, few specific protein markers, especially organ\u2010specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1\u2009Gy\u2010irradiated mice by employing 2\u2010DE analysis. MALDI\u2010TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR\u2010treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2\u2010DE data. These were heat shock protein 5 (HSP 5), HSP 90\u2009kDa \u03b2, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue\u2010specific protein markers of IR exposure.<\/jats:p>","DOI":"10.1002\/pmic.201000332","type":"journal-article","created":{"date-parts":[[2011,2,9]],"date-time":"2011-02-09T07:19:51Z","timestamp":1297235991000},"page":"1254-1263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Proteomic identification of radiation response markers in mouse intestine and brain"],"prefix":"10.1002","volume":"11","author":[{"given":"Young\u2010Bin","family":"Lim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u2010Jeong","family":"Pyun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae\u2010June","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u2010Rok","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeung Bae","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Sil","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,2,14]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"324","article-title":"Characterization of a novel epigenetic effect of ionizing radiation: the death\u2010inducing effect","volume":"63","author":"Nagar S.","year":"2003","journal-title":"Cancer Res."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200008-MCP200"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.1720"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1259\/0007-1285-42-501-688"},{"key":"e_1_2_6_6_2","first-page":"67","article-title":"Red marrow radiation dose adjustment using plasma FLT3\u2010L cytokine levels: improved correlations between hematologic toxicity and bone marrow dose for radioimmunotherapy patients","volume":"44","author":"Siegel J. 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