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In order to identify the candidate protein(s) responsible for the differential metastatic capacity, the protein expression profiling between NPC cell line CNE\u20102 and its highly metastatic subclone S\u201018 were compared by 2\u2010DE. In total, 18 spots were differentially expressed between these two cell lines. Among all, seven proteins were identified with further MS analysis. Western blotting further validated upregulation of HSP27 and ezrin, and downregulation of valosin containing protein and keratin 18 in S\u201018. Moreover, the knockdown of HSP27 was found to significantly decrease the invasive ability of S\u201018. On the other hand, overexpression of HSP27 in NP460 cells, which generated little endogenous HSP27 and less invasive, was noted to gain enhanced metastatic capability. Real\u2010time PCR confirmed that the transcriptional levels of NF\u2010\u03baB and MMP9, MMP11 were downregulated after inhibition of HSP27 in S\u201018, which implicated that HSP27 enhanced the metastatic property of NPC cells probably via the NF\u2010\u03baB\u2010mediated activation of MMPs. The findings in this work provided us a platform for further elucidating the underlying mechanisms of NPC metastasis and demonstrated that HSP27 would be a valid target for anti\u2010cancer drug development.<\/jats:p>","DOI":"10.1002\/pmic.201000483","type":"journal-article","created":{"date-parts":[[2011,5,17]],"date-time":"2011-05-17T06:23:08Z","timestamp":1305613388000},"page":"2911-2920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Proteomic profiling between CNE\u20102 and its strongly metastatic subclone S\u201018 and functional characterization of HSP27 in metastasis of nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"11","author":[{"given":"Guo\u2010Ping","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiu\u2010Kay","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Chun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie C. 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