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Here, we analyzed global protein expression patterns in plasma of control and sibutramine\u2010treated rats using proteomic analysis for a better understanding of the two conflicting functions of this drug, appetite regulation, and cardiovascular risk. The control (<jats:italic>n<\/jats:italic>=6) and sibutramine\u2010treated groups (<jats:italic>n<\/jats:italic>=6) were injected by vehicle and sibutramine, respectively, and 2\u2010DE combined with MALDI\u2010TOF\/MS were performed. Compared to control rats, sibutramine\u2010administered rats gained approximately 18% less body weight and consumed about 13% less food. Plasma leptin and insulin levels also showed a significant decrease in sibutramine\u2010treated rats. As a result of proteomic analysis, 23 differentially regulated proteins were discovered and were reconfirmed by immunoblot analysis. Changed proteins were classified into appetite regulation and cardiovascular risk, according to their regulation pattern. Because the differential levels of proteins that have been well recognized as predictors of CVD risk were not well matched with the results of our proteomic analysis, this study does not conclusively prove that sibutramine has an effect on CVD risk.<\/jats:p>","DOI":"10.1002\/pmic.201000664","type":"journal-article","created":{"date-parts":[[2011,2,2]],"date-time":"2011-02-02T11:33:41Z","timestamp":1296646421000},"page":"1300-1312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteome changes in rat plasma in response to sibutramine"],"prefix":"10.1002","volume":"11","author":[{"given":"Jung\u2010Won","family":"Choi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong In","family":"Joo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Seok","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duk Kwon","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Won","family":"Yun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,2,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2010.118"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27302009000200018"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03491-7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-142-7-200504050-00012"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0278-5846(88)90003-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90348-Y"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(00)00415-9"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(98)00032-X"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702446"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0800636"},{"key":"e_1_2_6_12_2","first-page":"675","article-title":"Sibutramine therapy in obese women \u2013 effects on plasma neuropeptide Y (NPY), insulin, leptin and beta\u2010endorphin concentrations","volume":"26","author":"Baranowska B.","year":"2005","journal-title":"Neuro Endocrinol. 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