{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:36:18Z","timestamp":1792647378521,"version":"4.5.0"},"reference-count":66,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T00:00:00Z","timestamp":1319760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Receptor tyrosine kinases (RTKs) are proteins that upon ligand stimulation undergo dimerization and autophosphorylation. Eph receptors (EphRs) are RTKs that are found in different cell types, from both tissues that are developing and from mature tissues, and play important roles in the development of the central nervous system and peripheral nervous system. EphRs also play roles in synapse formation, neural crest formation, angiogenesis and in remodeling the vascular system. Interaction of EphRs with their ephrin ligands lead to activation of signal transduction pathways and formation of many transient protein\u2013protein interactions that ultimately leads to cytoskeletal remodeling. However, the sequence of events at the molecular level is not well understood. We used blue native PAGE and MS to analyze the transient protein\u2013protein interactions that resulted from the stimulation of EphB2 receptors by their ephrinB1\u2010Fc ligands. We analyzed the phosphotyrosine\u2010containing protein complexes immunoprecipitated from the cell lysates of both unstimulated (\u2212) and ephrinB1\u2010Fc\u2010stimulated (+) NG108 cells. Our experiments allowed us to identify many signaling proteins, either known to be part of EphB2 signaling or new for this pathway, which are involved in transient protein\u2013protein interactions upon ephrinB1\u2010Fc stimulation. These data led us to investigate the roles of proteins such as FAK, WAVEs and Nischarin in EphB2 signaling.<\/jats:p>","DOI":"10.1002\/pmic.201000819","type":"journal-article","created":{"date-parts":[[2011,9,19]],"date-time":"2011-09-19T13:10:08Z","timestamp":1316437808000},"page":"4514-4528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Identifying transient protein\u2013protein interactions in EphB2 signaling by blue native PAGE and mass spectrometry"],"prefix":"10.1002","volume":"11","author":[{"given":"Costel C.","family":"Darie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Deinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helene S.","family":"Cardasis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moses V.","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Neubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/383722a0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(98)80015-9"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35058515"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00197-5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050931"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00137.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(01)00031-4"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050391m"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81426-9"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80276-7"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb823"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/35000008"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/08977190500055869"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5306.1640"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/35093123"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04685.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2007.10.011"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1112"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90094-A"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T300010-MCP200"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21174"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01511"},{"key":"e_1_2_6_24_2","first-page":"Unit 16.1","article-title":"Overview of peptide and protein analysis by mass spectrometry","author":"Zhang G.","year":"2010","journal-title":"Curr. 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